Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이성혁 | - |
dc.contributor.author | 윤환 | - |
dc.contributor.author | 강성균 | - |
dc.contributor.author | 이현숙 | - |
dc.date.accessioned | 2020-07-15T10:53:50Z | - |
dc.date.available | 2020-07-15T10:53:50Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2018-10-04 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/23107 | - |
dc.description.abstract | Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture with yeast extract as a sole energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species and the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins.energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species and the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | KIOST | - |
dc.relation.isPartOf | 2018 international workshop on deep-sea microbiology | - |
dc.title | Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 50 | - |
dc.citation.startPage | 50 | - |
dc.citation.title | 2018 international workshop on deep-sea microbiology | - |
dc.contributor.alternativeName | 이성혁 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.identifier.bibliographicCitation | 2018 international workshop on deep-sea microbiology, pp.50 | - |
dc.description.journalClass | 2 | - |