Hizikia fusiforme로부터 분리 된 Saringosterol acetate는 in vitro 및 in vivo zebrafish 모델에서 간세포 암종 억제

Abstract

Hizikia fusiforme, an edible brown alga widely consumed in Korea, Japan, and China have found to possess a number of potential biological functionalities. Current study encloses the potent anti-cancer effects of saringosterol acetate (SSA) isolated from the H. fusiforme towards the inhibitory effects on hepatocellular carcinoma in Hep3B cell. SSA markedly inhibited cancer cell growth, attenuating the population of cells in sub-G1 through PI3K/Akt/mTOR pathway. Further studies elaborate the therapeutic effects of SSA against diethylnitrosamin (DEN)-stimulated cancer in zebrafish model. DEN treatment caused a marked increase in reactive oxygen species (ROS) production, cell death and expression of TFGβ and MMP2 in zebrafish. However, treatment of SSA significantly downregulated DEN-induced ROS, cell death and TFGβ and MMP2 expressions. These results exhibit the potential anticancer effects of SSA and highlight its potential applications related to nutraceuticals or functional food to reduced carcinogenic effect. Further this highlights the suitability of zebrafish in vivo model to mimic cancer for evaluating the reduce hepatocellular carcinogenic effects properties of material. isolated from the H. fusiforme towards the inhibitory effects on hepatocellular carcinoma in Hep3B cell. SSA markedly inhibited cancer cell growth, attenuating the population of cells in sub-G1 through PI3K/Akt/mTOR pathway. Further studies elaborate the therapeutic effects of SSA against diethylnitrosamin (DEN)-stimulated cancer in zebrafish model. DEN treatment caused a marked increase in reactive oxygen species (ROS) production, cell death and expression of TFGβ and MMP2 in zebrafish. However, treatment of SSA significantly downregulated DEN-induced ROS, cell death and TFGβ and MMP2 expressions. These results exhibit the potential anticancer effects of SSA and highlight its potential applications related to nutraceuticals or functional food to reduced carcinogenic effect. Further this highlights the suitability of zebrafish in vivo model to mimic cancer for evaluating the reduce hepatocellular carcinogenic effects properties of material.