Red Seacucumber (Stichopus japonicus) Alleviates LPS-induced Inflammatory Response through ROS and MAPK Pathways in RAW264.7 Macrophages

Title
Red Seacucumber (Stichopus japonicus) Alleviates LPS-induced Inflammatory Response through ROS and MAPK Pathways in RAW264.7 Macrophages
Author(s)
김은아; 김준성; 허수진
KIOST Author(s)
Kim, Eun A(김은아)Kim, Junseong(김준성)Heo, Soo Jin(허수진)
Publication Year
2018-11-28
Abstract
Seacucmbers are used in formulation of traditional medicine in east asia and it was known that it exhibits different activities depending on the habitat. Therefore, in this study, we confirmed the anti-inflammatory effect of red seacucumber (Stichopus japonicus) from Jeju Island. We obtained a vacuum dried S. japonicas extracts (VDSEs) by treatment with MeOH or MeOH/DCM (2:1). Both extractions showed inhibition of NO production without toxicity. And VDSEs significantly inhibited PGE2, protein level of iNOS and COX-2 as well as pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α at 20 and 50 μg/ml. VDSEs suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK, JNK, and p38 also reduced ROS generation. Our results demonstrated that S. japonicas extracts using different extraction methods presented anti-inflammatory activity by inhibiting MAPKs and ROS generation. Therefore, VDSEs can be used and applied as a useful anti-inflammatory agent. (Stichopus japonicus) from Jeju Island. We obtained a vacuum dried S. japonicas extracts (VDSEs) by treatment with MeOH or MeOH/DCM (2:1). Both extractions showed inhibition of NO production without toxicity. And VDSEs significantly inhibited PGE2, protein level of iNOS and COX-2 as well as pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α at 20 and 50 μg/ml. VDSEs suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK, JNK, and p38 also reduced ROS generation. Our results demonstrated that S. japonicas extracts using different extraction methods presented anti-inflammatory activity by inhibiting MAPKs and ROS generation. Therefore, VDSEs can be used and applied as a useful anti-inflammatory agent.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22849
Bibliographic Citation
한국식품영양과학회, pp.52, 2018
Publisher
한국식품영양과학회
Type
Conference
Language
English
Publisher
한국식품영양과학회
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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