인도양 중앙해령 열수구의 미생물 다양성 분석 및 동정

Abstract

Through 2018 Indian Ocean hydrothermal vent exploration, we obtained samples from the previously known as hydrothermal vent areas, Edmond and Solitaire, and from the newly discovered OVF (Onnuri Vent Field) site. Microbial diversity near suspected hydrothermal vent area had been analyzed from above sites through the amplicon sequencing approach. The communities found in Edmond site were dominated by Thermodesulfovibrio and Campylonacterales order. In Solitaire site, Thiotricales and Campylonacterales order were abundant. The newly discovered OVF area showed high proportion of Chromatiales and Actinomarinales order. In addition, enrichment cultures for isolation of chemolithoautotrophs and hyperthermophiles under mesophilic, thermophilic and hyperthermophilic condition has been progress. We have isolated sulfur-oxidizing, autotrophic microorganisms, Sulfurimonas and Sulfurovum species. Also, we have obtained 5 strains belonging to the hyperthermophilic archaeon, Thermococcus genus. Currently, we are analyzing the physiological characteristics of the obtained strains.