Effects of booster biocides and by-products from vessels on embryo test of a sea urchin, Mesocentrotus nudus

Abstract

In this study, we aimed to assess the toxicity of biocide including of antifouling paint and by-products from vessels on embryos of a sea urchin, Mesocentrotus nudus. In this study evaluated toxicity on the 5 kinds of biocide (Sea-nine 211, Diuron, Irgarol 1051, CuPT, ZnPT), water jet from R/V EARDO and ISABU in KIOST, and water jet extracted with MeOH. As a result of the fertilization rate of sea urchin for 5 kinds of biocide, the value of EC50 of Sea-nine was 8.134 ㎍/L, but those of Diuron and Irgarol were 10,777 ㎍/L and 4,476 ㎍/L, respectively. The value of EC50 of CuPT and ZnPT were 3.423 ㎍/L and 6.488 ㎍/L, respectively. Among 5 AFS active substances, the highest toxicity was found in CuPT. To assess the adverse effects of the wastes on marine biota from ship’s surface during in-water cleaning activities, we conducted the toxicity test for by-products using sea urchin embryos. The fertilization rate of sea urchin embryos exposed on thewater samples from the water jet operation on the R/V EARDO was 79±5.8% at 1000-fold diluted solution, and the fertilization rates exposed in 10-fold and 100-fold diluted solutions were 0.2±0.5% and 2.3±1.4%, respectively. The fertilization rate in the water jet samples from the R/V ISABU was 0.2% in 10-fold diluted solutions, but normal fertilization was found (>90%) in 100-fold diluted solution. The fertilization rate on the MeOH extracted solution of wastes from the R/V