Saringosterol acetae Inhibits Liver Cancer Progression via PI3K/Akt/mTOR Signaling in A Zerafish Xenograft model

Abstract

Background : Hizikia fusiforme has been found to possess a number of potential biological functionalities but just a few reports have revealed for the relationship between activities and compounds. The zebrafish model system is one of the most widely used animal models. That is attaining popularity as becomes an attractive model for molecular genetics, developmental biology, drug discovery and screening of human disease. In this study, we established that inhibition of livercancer progression effects was occurred in human cancer cell xenograft zebrafish model. Methods : The zebrafish were injected with saringosterol acetate (SSA) isolated from Hizikia fusiforme every three days. After a week, the abdominal cavity of zebrafish was inoculated with Hep3B cells during ten times a month.Results : Angiogenic factors (VEGF, TGFβ, MMP2, MMP9, IL6, and TNF-α), and α-fetoprotien (AFP) production were significantly increased in HepB cells-injected group compared with normal group. However, SSA considerably inhibited angiogenic factors and AFP production. Further mechanistic studies showed that SSA-injected group suppressed TGFβ pathways and phosphorylation of PI3k/Akt/mTOR pathways in the liver tissues treated with SSA