Stichopus japonicus Suppresses Inflammatory and Cancer Progression by Promoting ROS-Mediated Inhibition of MAPK Signaling.

Title
Stichopus japonicus Suppresses Inflammatory and Cancer Progression by Promoting ROS-Mediated Inhibition of MAPK Signaling.
Author(s)
김준성; 김은아; 강나래; 강도형; 허수진
KIOST Author(s)
Kim, Junseong(김준성)Kim, Eun A(김은아)Kang, Nalae(강나래)Kang, Do-Hyung(강도형)Heo, Soo Jin(허수진)
Publication Year
2019-05-01
Abstract
Background : Sea cucmbers are used in formulation of traditional medicine in east asia and it was known that it exhibits different activities depending on the habitat. Therefore, in this study, we confirmed the anti-inflammatory and anti-cancer effect of red sea cucumber (Stichopus japonicus) from Jeju Island. Methods : We obtained a vacuum dried S. japonicas extracts by treatment with MeOH (VD-M) or MeOH/DCM (2:1) (VD-D). VD-M and VD-D used experiment to ROS generation and protein expression. Result : Both extractions showed inhibition of NO production without toxicity. And VD-M and VD-D significantly inhibited PGE2, protein level of iNOS and COX-2 as well as pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α. VD-M and VD-D suppressed the phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as ERK, JNK, and p38 also reduced ROS generation. Our results demonstrated that S. japonicas extracts using different extraction methods presented anti-inflammatory activity by inhibiting MAPKs and ROS generation. VD-M showed inhibition of tumor cell growth and induced increased ROS generation which is associated with the induction of apoptosis though mitogen-activated protein kinase (MAPK) pathway in murine colon carcinoma cells (CT-26). The combination of VD-M and N-acetylcysteine (NAC), a ROS scavenger, increased ROS production and apoptosis compare with non-treated group via regulation MA
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22738
Bibliographic Citation
International Seaweed Symposium, pp.177, 2019
Publisher
The Korean Society of Phycology
Type
Conference
Language
English
Publisher
The Korean Society of Phycology
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse