Biohydrogen Production of Obligate Anaerobic Archaeon Thermococcus onnurineus NA1 under Oxic Conditions via Overexpression of frhAGB-encoding Hydrogenase Genes

Title
Biohydrogen Production of Obligate Anaerobic Archaeon Thermococcus onnurineus NA1 under Oxic Conditions via Overexpression of frhAGB-encoding Hydrogenase Genes
Author(s)
이성혁; 김민식; 강성균; 이현숙
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2019-06-24
Abstract
In order to understand the physiological role of a soluble hydrogenase gene cluster, frhAGB, in a hyperthermophilic archaeon T. onnurineus NA1, we tried to understand the changes in the mutant by employing transcriptome analysis. The overexpression of frhAGB genes caused changing expression of several genes involved in stress response and transcriptional regulation. We revealed that no significant growth defect was observed when the frhAGB-overexpressing mutant (FO) was exposed to 40% air and enable grow on 20% O2 in the headspace of sealed bottle. Furthermore, FO mutant can grow and produce H2 in a bioreactor by omitting O2 removal methods. On the other hand, no growth was occurred in wild-type strain under oxic condition. This study gives an insight into the function of the frhAGB hydrogenase in T. onnurineus NA1 and we could demonstrate H2 production without oxygen removal of obligate anaerobic hyperthermophilic archaeon by overexpression of frhAGB hydrogenase.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22574
Bibliographic Citation
한국미생물생명공학회, pp.95, 2019
Publisher
한국미생물생명공학회
Type
Conference
Language
English
Publisher
한국미생물생명공학회
Related Researcher
Research Interests

Marine extremophile,해양극한미생물

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