Rapid Detection of Cochlodinium polykrikoides by Sandwich Hybridization Integrated with Invertase Assay

Title
Rapid Detection of Cochlodinium polykrikoides by Sandwich Hybridization Integrated with Invertase Assay
Author(s)
김강은; 강민경; 박미례; 이택견
KIOST Author(s)
Kim, Kangeun(김강은)Lee, Taek Kyun(이택견)
Publication Year
2019-10-24
Abstract
Rapid and accurate identification of marine microalgae causing harmful algal blooms (HABs) is a very important tool for predicting and managing HABs. We previously developed a nuclease protection assay sandwich hybridization (NPA-SH) method for the in situ detection of blooming microalgae Cochlodinium polykrikoides. In this study, we improved the applicability of the NPA-SH method for the detection of C. polykrikoides by simplifying the reaction step. For this purpose, invertase (INV) was conjugated to signal probe instead of using fluorescence, and sucrose was used as a reactant to induce a color reaction. The INV-signal probe conjugation was confirmed by SDS-PAGE and epifluoromicroscopy, and the amounts of the appropriate probe and sucrose, and the treatment time for optimizing the reaction were determined. As a result, the developed INV-SH reduced the treatment time in the field compared with NPA-SH, and it was possible to use a relatively small volume and low-priced personal glucose meter as well as an absorbance meter. INV-SH is the first C. polykrikoides species identification technology to which INV has been applied and could be an improved field technique.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22222
Bibliographic Citation
한국조류학회 학술발표대회 및 정기총회, pp.144, 2019
Publisher
한국조류학회
Type
Conference
Language
English
Publisher
한국조류학회
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