Optimization of up- and down-stream processes for microalga-based mammalian cells culture media

DC Field Value Language
dc.contributor.author 박아름이 -
dc.contributor.author 정윤식 -
dc.contributor.author 이연지 -
dc.contributor.author 김태호 -
dc.contributor.author 이원규 -
dc.contributor.author 유용균 -
dc.contributor.author 박건후 -
dc.contributor.author 이수진 -
dc.contributor.author 강도형 -
dc.date.accessioned 2020-07-15T06:51:01Z -
dc.date.available 2020-07-15T06:51:01Z -
dc.date.created 2020-02-11 -
dc.date.issued 2019-10-24 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/22208 -
dc.description.abstract FBS is the most widely used serum-supplement for in vitro cell culture of animal cell lines. However, sustainability of FBS industry has aware of ethical, economics and environmental issues despite the serum is important in the field of worldwide cell industry. The development of the serum-substituted media is being remarkable with strong interest in worldwide. In this study, we designed for optimization processes from upstream to downstream in order to make a FBS-alternative solution using in situ cultured Spirulina maxima biomass. S. maxima were cultured quarterly using by ROSE-max culture system, and were used as a raw material for manufacturing Spirulina Animal Cell Culture Solution (denoted as SACCS). Thepreprocessing method of SACCS was established by the processes via S. maxima cell milking, extraction and filters system. In the results, quality of the serial SACCS production based on Lot# was confirmed by contamination of mycoplasma, fungi and bacteria by PCR, and then all of the samples were not detected. Endotoxin test showed safe level below 5 EU/ml according to the guideline of ISIA (the International Serum Industry Association). Protein, carbohydrate, fat and minerals were analyzed and presented. Heavy metals were insignificantly detected by the inorganic analysis. For identifying adaptable cells culture, T24 (human urinary bladder cancer model) and H358 (lung cancer tumor model) cells were then applied -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국조류학회 -
dc.relation.isPartOf 한국조류학회 -
dc.title Optimization of up- and down-stream processes for microalga-based mammalian cells culture media -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 148 -
dc.citation.startPage 148 -
dc.citation.title 한국조류학회 -
dc.contributor.alternativeName 박아름이 -
dc.contributor.alternativeName 정윤식 -
dc.contributor.alternativeName 이연지 -
dc.contributor.alternativeName 김태호 -
dc.contributor.alternativeName 이원규 -
dc.contributor.alternativeName 유용균 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation 한국조류학회, pp.148 -
dc.description.journalClass 2 -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 2. Conference Papers
Jeju Research Institute > Tropical & Subtropical Research Center > 2. Conference Papers
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