Optimization of up- and down-stream processes for microalga-based mammalian cells culture media

Title
Optimization of up- and down-stream processes for microalga-based mammalian cells culture media
Author(s)
박아름이; 정윤식; 이연지; 김태호; 이원규; 유용균; 박건후; 이수진; 강도형
KIOST Author(s)
Park, Areumi(박아름이)Jeong, Younsik(정윤식)Lee, Yeon-Ji(이연지)Kim, Taeho(김태호)Lee, Won Kyu(이원규)Ryu, Yong Kyun(유용균)Kang, Do-Hyung(강도형)
Publication Year
2019-10-24
Abstract
FBS is the most widely used serum-supplement for in vitro cell culture of animal cell lines. However, sustainability of FBS industry has aware of ethical, economics and environmental issues despite the serum is important in the field of worldwide cell industry. The development of the serum-substituted media is being remarkable with strong interest in worldwide. In this study, we designed for optimization processes from upstream to downstream in order to make a FBS-alternative solution using in situ cultured Spirulina maxima biomass. S. maxima were cultured quarterly using by ROSE-max culture system, and were used as a raw material for manufacturing Spirulina Animal Cell Culture Solution (denoted as SACCS). Thepreprocessing method of SACCS was established by the processes via S. maxima cell milking, extraction and filters system. In the results, quality of the serial SACCS production based on Lot# was confirmed by contamination of mycoplasma, fungi and bacteria by PCR, and then all of the samples were not detected. Endotoxin test showed safe level below 5 EU/ml according to the guideline of ISIA (the International Serum Industry Association). Protein, carbohydrate, fat and minerals were analyzed and presented. Heavy metals were insignificantly detected by the inorganic analysis. For identifying adaptable cells culture, T24 (human urinary bladder cancer model) and H358 (lung cancer tumor model) cells were then applied
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22208
Bibliographic Citation
한국조류학회, pp.148, 2019
Publisher
한국조류학회
Type
Conference
Language
English
Publisher
한국조류학회
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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