A novel family VIII carboxylesterase hydrolysing third- and fourth-generation cephalosporins SCIE SCOPUS

Cited 7 time in WEB OF SCIENCE Cited 10 time in Scopus
Title
A novel family VIII carboxylesterase hydrolysing third- and fourth-generation cephalosporins
Author(s)
Jeon, Jeong Ho; Lee, Hyun Sook; Lee, Jung Hun; Koo, Bon-Sung; Lee, Chang-Muk; Lee, Sang Hee; Kang, Sung Gyun; Lee, Jung-Hyun
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2016-04
Abstract
A metagenomic library was constructed from a soil sample of spindle tree-rhizosphere. From this library, one clone with esterase activity was selected. The sequence analysis revealed an open reading frame (EstSTR1) encoded protein of 390 amino acids. EstSTR1 is a family VIII carboxylesterase and retains the S-X-X-K motif conserved in both family VIII carboxylesterases and class C beta-lactamases. The estSTR1 gene was overexpressed in E. coli and the recombinant protein was purified by purified by metal chelating affinity chromatography and size-exclusion chromatography. EstSTR1 hydrolysed p-nitrophenyl esters, exhibited the highest activity toward p-nitrophenyl butyrate. Furthermore, EstSTR1 could hydrolyse third-and fourth-generation cephalosporins (cefotaxime and cefepime) as well as first-generation cephalosporin (cephalothin). Site-directed mutagenesis studies revealed that a catalytic residue, Ser71, of EstSTR1 plays an essential role in hydrolysing both antibiotics and p-nitrophenyl esters. We demonstrate that a metagenome-derived carboxylesterase displays beta-lactam-hydrolysing activities toward third- and fourth-generation cephalosporins.
ISSN
2193-1801
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2206
DOI
10.1186/s40064-016-2172-y
Bibliographic Citation
SPRINGERPLUS, v.5, 2016
Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
Keywords
Metagenome; beta-Lactamase; Carboxylesterase; Extended-spectrum cephalosporins
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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