5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages SCIE SCOPUS

DC Field Value Language
dc.contributor.author Kim, Kil-Nam -
dc.contributor.author Ko, Seok-Chun -
dc.contributor.author Ye, Bo-Ram -
dc.contributor.author Kim, Min-Sun -
dc.contributor.author Kim, Junseong -
dc.contributor.author Ko, Eun-Yi -
dc.contributor.author Cho, Su-Hyeon -
dc.contributor.author Kim, Daekyung -
dc.contributor.author Heo, Soo-Jin -
dc.contributor.author Jung, Won-Kyo -
dc.date.accessioned 2020-04-20T02:25:38Z -
dc.date.available 2020-04-20T02:25:38Z -
dc.date.created 2020-01-28 -
dc.date.issued 2016-10-25 -
dc.identifier.issn 0009-2797 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2151 -
dc.description.abstract The aim of the present study was to investigate the effects of 5-bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) on inflammatory responses to lipopolysaccharide (LPS) in RAW 264.7 cells and the associated mechanism of action. BHMB concentration-dependently suppressed protein and mRNA expressions of iNOS and COX-2, thereby inhibiting the production of NO and PGE(2) in LPS-stimulated RAW 264.7 cells. BHMB also reduced the mRNA expression of TNF-alpha, IL-6, and IL-1 beta in LPS-stimulated RAW 264.7 cells. To elucidate the mechanism underlying the anti-inflammatory activity of BHMB, we investigated the effects of BHMB on the mitogen-activated protein kinase and nuclear factor-kappa B (NF-kappa B) pathways. BHMB suppressed the phosphorylation and degradation of I kappa B-alpha and markedly inhibited the nuclear translocation of p65 and p50 in LPS-stimulated RAW 264.7 cells. The compound also inhibited the LPS-stimulated phosphorylation of ERK and p38. Taken together, these results illustrated that BHMB suppresses pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 cells by inhibiting the phosphorylation of ERK and p38 and the activation of NF-kappa B. (C) 2016 Elsevier Ireland Ltd. All rights reserved. -
dc.description.uri 1 -
dc.language English -
dc.publisher ELSEVIER IRELAND LTD -
dc.subject NITRIC-OXIDE SYNTHASE -
dc.subject ACTIVATED PROTEIN-KINASE -
dc.subject MAMMALIAN-CELLS -
dc.subject CAFFEIC ACID -
dc.subject BACTERIAL LIPOPOLYSACCHARIDE -
dc.subject PHENOLIC-COMPOUNDS -
dc.subject INTERFERON-GAMMA -
dc.subject GENE-EXPRESSION -
dc.subject IN-VIVO -
dc.subject CYCLOOXYGENASE-2 -
dc.title 5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages -
dc.type Article -
dc.citation.endPage 114 -
dc.citation.startPage 108 -
dc.citation.title CHEMICO-BIOLOGICAL INTERACTIONS -
dc.citation.volume 258 -
dc.contributor.alternativeName 예보람 -
dc.contributor.alternativeName 김민선 -
dc.contributor.alternativeName 김준성 -
dc.contributor.alternativeName 허수진 -
dc.identifier.bibliographicCitation CHEMICO-BIOLOGICAL INTERACTIONS, v.258, pp.108 - 114 -
dc.identifier.doi 10.1016/j.cbi.2016.08.022 -
dc.identifier.scopusid 2-s2.0-84988474063 -
dc.identifier.wosid 000385368700012 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus NITRIC-OXIDE SYNTHASE -
dc.subject.keywordPlus ACTIVATED PROTEIN-KINASE -
dc.subject.keywordPlus MAMMALIAN-CELLS -
dc.subject.keywordPlus CAFFEIC ACID -
dc.subject.keywordPlus BACTERIAL LIPOPOLYSACCHARIDE -
dc.subject.keywordPlus PHENOLIC-COMPOUNDS -
dc.subject.keywordPlus INTERFERON-GAMMA -
dc.subject.keywordPlus GENE-EXPRESSION -
dc.subject.keywordPlus IN-VIVO -
dc.subject.keywordPlus CYCLOOXYGENASE-2 -
dc.subject.keywordAuthor 5-Bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) -
dc.subject.keywordAuthor Proinflammatory -
dc.subject.keywordAuthor RAW 264.7 cells -
dc.subject.keywordAuthor MAPKs -
dc.subject.keywordAuthor NF-kappa B -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalWebOfScienceCategory Pharmacology & Pharmacy -
dc.relation.journalWebOfScienceCategory Toxicology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Pharmacology & Pharmacy -
dc.relation.journalResearchArea Toxicology -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 1. Journal Articles
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
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