5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Kil-Nam | - |
dc.contributor.author | Ko, Seok-Chun | - |
dc.contributor.author | Ye, Bo-Ram | - |
dc.contributor.author | Kim, Min-Sun | - |
dc.contributor.author | Kim, Junseong | - |
dc.contributor.author | Ko, Eun-Yi | - |
dc.contributor.author | Cho, Su-Hyeon | - |
dc.contributor.author | Kim, Daekyung | - |
dc.contributor.author | Heo, Soo-Jin | - |
dc.contributor.author | Jung, Won-Kyo | - |
dc.date.accessioned | 2020-04-20T02:25:38Z | - |
dc.date.available | 2020-04-20T02:25:38Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2016-10-25 | - |
dc.identifier.issn | 0009-2797 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2151 | - |
dc.description.abstract | The aim of the present study was to investigate the effects of 5-bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) on inflammatory responses to lipopolysaccharide (LPS) in RAW 264.7 cells and the associated mechanism of action. BHMB concentration-dependently suppressed protein and mRNA expressions of iNOS and COX-2, thereby inhibiting the production of NO and PGE(2) in LPS-stimulated RAW 264.7 cells. BHMB also reduced the mRNA expression of TNF-alpha, IL-6, and IL-1 beta in LPS-stimulated RAW 264.7 cells. To elucidate the mechanism underlying the anti-inflammatory activity of BHMB, we investigated the effects of BHMB on the mitogen-activated protein kinase and nuclear factor-kappa B (NF-kappa B) pathways. BHMB suppressed the phosphorylation and degradation of I kappa B-alpha and markedly inhibited the nuclear translocation of p65 and p50 in LPS-stimulated RAW 264.7 cells. The compound also inhibited the LPS-stimulated phosphorylation of ERK and p38. Taken together, these results illustrated that BHMB suppresses pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 cells by inhibiting the phosphorylation of ERK and p38 and the activation of NF-kappa B. (C) 2016 Elsevier Ireland Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | CAFFEIC ACID | - |
dc.subject | BACTERIAL LIPOPOLYSACCHARIDE | - |
dc.subject | PHENOLIC-COMPOUNDS | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IN-VIVO | - |
dc.subject | CYCLOOXYGENASE-2 | - |
dc.title | 5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages | - |
dc.type | Article | - |
dc.citation.endPage | 114 | - |
dc.citation.startPage | 108 | - |
dc.citation.title | CHEMICO-BIOLOGICAL INTERACTIONS | - |
dc.citation.volume | 258 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.contributor.alternativeName | 김준성 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | CHEMICO-BIOLOGICAL INTERACTIONS, v.258, pp.108 - 114 | - |
dc.identifier.doi | 10.1016/j.cbi.2016.08.022 | - |
dc.identifier.scopusid | 2-s2.0-84988474063 | - |
dc.identifier.wosid | 000385368700012 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | CAFFEIC ACID | - |
dc.subject.keywordPlus | BACTERIAL LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | PHENOLIC-COMPOUNDS | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 | - |
dc.subject.keywordAuthor | 5-Bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) | - |
dc.subject.keywordAuthor | Proinflammatory | - |
dc.subject.keywordAuthor | RAW 264.7 cells | - |
dc.subject.keywordAuthor | MAPKs | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |