5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages SCIE SCOPUS

Cited 14 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
5-Bromo-2-hydroxy-4-methyl-benzaldehyde inhibited LPS-induced production of pro-inflammatory mediators through the inactivation of ERK, p38, and NF-kappa B pathways in RAW 264.7 macrophages
Author(s)
Kim, Kil-Nam; Ko, Seok-Chun; Ye, Bo-Ram; Kim, Min-Sun; Kim, Junseong; Ko, Eun-Yi; Cho, Su-Hyeon; Kim, Daekyung; Heo, Soo-Jin; Jung, Won-Kyo
KIOST Author(s)
Kim, Junseong(김준성)Heo, Soo Jin(허수진)
Publication Year
2016-10-25
Abstract
The aim of the present study was to investigate the effects of 5-bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) on inflammatory responses to lipopolysaccharide (LPS) in RAW 264.7 cells and the associated mechanism of action. BHMB concentration-dependently suppressed protein and mRNA expressions of iNOS and COX-2, thereby inhibiting the production of NO and PGE(2) in LPS-stimulated RAW 264.7 cells. BHMB also reduced the mRNA expression of TNF-alpha, IL-6, and IL-1 beta in LPS-stimulated RAW 264.7 cells. To elucidate the mechanism underlying the anti-inflammatory activity of BHMB, we investigated the effects of BHMB on the mitogen-activated protein kinase and nuclear factor-kappa B (NF-kappa B) pathways. BHMB suppressed the phosphorylation and degradation of I kappa B-alpha and markedly inhibited the nuclear translocation of p65 and p50 in LPS-stimulated RAW 264.7 cells. The compound also inhibited the LPS-stimulated phosphorylation of ERK and p38. Taken together, these results illustrated that BHMB suppresses pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 cells by inhibiting the phosphorylation of ERK and p38 and the activation of NF-kappa B. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
ISSN
0009-2797
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2151
DOI
10.1016/j.cbi.2016.08.022
Bibliographic Citation
CHEMICO-BIOLOGICAL INTERACTIONS, v.258, pp.108 - 114, 2016
Publisher
ELSEVIER IRELAND LTD
Subject
NITRIC-OXIDE SYNTHASE; ACTIVATED PROTEIN-KINASE; MAMMALIAN-CELLS; CAFFEIC ACID; BACTERIAL LIPOPOLYSACCHARIDE; PHENOLIC-COMPOUNDS; INTERFERON-GAMMA; GENE-EXPRESSION; IN-VIVO; CYCLOOXYGENASE-2
Keywords
5-Bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB); Proinflammatory; RAW 264.7 cells; MAPKs; NF-kappa B
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER IRELAND LTD
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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