Chitosan based silver nanocomposites (CAgNCs) display antibacterial effects against Vibrio ichthyoenteri SCOPUS KCI

Title
Chitosan based silver nanocomposites (CAgNCs) display antibacterial effects against Vibrio ichthyoenteri
Author(s)
Beom, S.S.; Shin, S.Y.; Dananjaya, S.H.S.; De Silva, A.B.K.H.; Nikapitiya, C.; Cho, J.; Park, G.-H.; Oh, C.; Kang, D.-H.; De Zoysa, M.
KIOST Author(s)
Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)
Publication Year
2017
Abstract
The aim of this study was to investigate the antibacterial properties of chitosan silver nanocomposites (CAgNCs) using pathogenic Vibrio ichthyoenteri as a bacterial model. Results of agar disc diffusion and turbidimetric assays showed that CAgNCs could inhibit the growth of V. ichthyoenteri in concentration dependent manner. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of CAgNCs were 75 and 125 µg/mL, respectively. Furthermore, CAgNCs treatment induced the reactive oxygen species (ROS) level in V. ichthyoenteri cells in concentration and time dependent manner, suggesting that it generates oxidative stress, leading to bacterial cell death. The field emission scanning electron microscope (FE-SEM) images of CAgNCs treated V. ichthyoenteri exhibited strong cell membrane damage than un-treated control bacteria. MTT assay results showed the highest cell viability (22%) at 75 µg/mL of CAgNCs treated bacteria samples. The results from this study suggest that CAgNCs is a potential antibacterial agent to control fish pathogenic bacteria. © 2017, Korean Society of Veterinary Clinics. All rights reserved.
ISSN
1598-298X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2135
DOI
10.17555/jvc.2017.08.34.4.261
Bibliographic Citation
Journal of Veterinary Clinics, v.34, no.4, pp.261 - 267, 2017
Publisher
Korean Society of Veterinary Clinics
Subject
chitosan; nanocomposite; propidium iodide; reactive oxygen metabolite; silver; antibacterial activity; Article; bacterial cell; cell membrane; cell membrane permeability; cell viability; cytotoxicity; field emission; flow cytometry; fluorescence; membrane damage; minimum bactericidal concentration; minimum inhibitory concentration; MTT assay; nonhuman; oxidative stress; Vibrio
Keywords
Antimicrobial agents; Chitosan-silver nanocomposites; Vibrio ichthyoenteri
Type
Article
Language
English
Document Type
Article
Publisher
Korean Society of Veterinary Clinics
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