Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1
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Title
- Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1
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Author(s)
- Yun, Sung Ho; Lee, Sang-Yeop; Choi, Chi-Won; Lee, Hayoung; Ro, Hyun-Joo; Jun, Sangmi; Kwon, Yong Min; Kwon, Kae Kyoung; Kim, Sang-Jin; Kim, Gun-Hwa; Kim, Seung Il
- KIOST Author(s)
- Kwon, Kae Kyoung(권개경)
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Alternative Author(s)
- 권개경; 김상진
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Publication Year
- 2017-01
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Abstract
- Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several poly cyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo [a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 mn. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane-associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.
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ISSN
- 1225-8873
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2133
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DOI
- 10.1007/s12275-017-6581-6
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Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.55, no.1, pp.56 - 62, 2017
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Publisher
- MICROBIOLOGICAL SOCIETY KOREA
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Subject
- PSEUDOMONAS-PUTIDA KT2440; ESCHERICHIA-COLI; GENOME SEQUENCE; PREDICTION; PROTEINS
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Keywords
- Novosphingobium pentaromativorans; outer membrane vesicle; proteomics
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Type
- Article
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Language
- English
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Document Type
- Article
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