Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1 SCIE SCOPUS KCI

Cited 9 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1
Author(s)
Yun, Sung Ho; Lee, Sang-Yeop; Choi, Chi-Won; Lee, Hayoung; Ro, Hyun-Joo; Jun, Sangmi; Kwon, Yong Min; Kwon, Kae Kyoung; Kim, Sang-Jin; Kim, Gun-Hwa; Kim, Seung Il
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Publication Year
2017-01
Abstract
Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several poly cyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo [a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 mn. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane-associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.
ISSN
1225-8873
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2133
DOI
10.1007/s12275-017-6581-6
Bibliographic Citation
JOURNAL OF MICROBIOLOGY, v.55, no.1, pp.56 - 62, 2017
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Subject
PSEUDOMONAS-PUTIDA KT2440; ESCHERICHIA-COLI; GENOME SEQUENCE; PREDICTION; PROTEINS
Keywords
Novosphingobium pentaromativorans; outer membrane vesicle; proteomics
Type
Article
Language
English
Document Type
Article
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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