Purification and molecular docking study of angiotensin I-converting enzyme (ACE) inhibitory peptides from hydrolysates of marine sponge Stylotella aurantium SCIE SCOPUS

Cited 22 time in WEB OF SCIENCE Cited 22 time in Scopus
Title
Purification and molecular docking study of angiotensin I-converting enzyme (ACE) inhibitory peptides from hydrolysates of marine sponge Stylotella aurantium
Author(s)
Ko, Seok-Chun; Jang, Jiyi; Ye, Bo-Ram; Kim, Min-Sun; Choi, Il-Whan; Park, Won-Sun; Heo, Soo-Jin; Jung, Won-Kyo
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2017-03
Abstract
Angioteinsin I-converting enzyme (ACE) inhibitory peptide was isolated from marine sponge (Stylotella aurantium) hydrolysate prepared by various hydrolysis enzymes. The peptic hydrolysate exhibited highest ACE inhibitory activity among them and was fractionated into three ranges of molecular weight. The below 5 kDa fraction showed the highest ACE inhibitory activity and was used for subsequent purification steps. The amino acid sequences of the purified peptides were identified to be Tyr-Arg (337.2 Da), and Ile-Arg (287.2 Da). The purified peptides from marine sponge had an IC50 value of 237.2 mu M and 306.4 mu M, respectively. The molecular docking study revealed that ACE inhibitory activity of the purified peptides was mainly attributed to the hydrogen bond interactions and Pi interaction between the dipeptides and ACE. The results suggest that marine sponge, S. aurantium would be an attractive raw material for the manufacture of anti-hypertensive nutraceutical ingredients. (C) 2016 Elsevier Ltd. All rights reserved.
ISSN
1359-5113
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2128
DOI
10.1016/j.procbio.2016.12.023
Bibliographic Citation
PROCESS BIOCHEMISTRY, v.54, pp.180 - 187, 2017
Publisher
ELSEVIER SCI LTD
Subject
SPONTANEOUSLY HYPERTENSIVE-RATS; PROTEIN HYDROLYSATE; BIOCHEMICAL-CHARACTERIZATION; FRAME PROTEIN; SKIN; IDENTIFICATION
Keywords
Marine sponge; Stylotella aurantium; Angioteinsin I-converting enzyme (ACE); Molecular docking; Peptides
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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