A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria SCIE SCOPUS KCI

DC Field Value Language
dc.contributor.author Hettiarachchi, Sachithra Amarin -
dc.contributor.author Lee, Su-Jin -
dc.contributor.author Lee, Youngdeuk -
dc.contributor.author Kwon, Young-Kyung -
dc.contributor.author De Zoysa, Mahanama -
dc.contributor.author Moon, Song -
dc.contributor.author Jo, Eunyoung -
dc.contributor.author Kim, Taeho -
dc.contributor.author Kang, Do-Hyung -
dc.contributor.author Heo, Soo-Jin -
dc.contributor.author Oh, Chulhong -
dc.date.accessioned 2020-04-20T02:25:18Z -
dc.date.available 2020-04-20T02:25:18Z -
dc.date.created 2020-01-28 -
dc.date.issued 2017-08 -
dc.identifier.issn 1017-7825 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2120 -
dc.description.abstract Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria. -
dc.description.uri 1 -
dc.language English -
dc.publisher KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY -
dc.subject SP-NOV. -
dc.subject MARINE-BACTERIA -
dc.subject ANTIMICROBIAL ACTIVITY -
dc.subject BACILLUS-SUBTILIS -
dc.subject NATURAL-PRODUCTS -
dc.subject CAPACITY -
dc.subject CEREUS -
dc.title A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria -
dc.type Article -
dc.citation.endPage 1448 -
dc.citation.startPage 1441 -
dc.citation.title JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY -
dc.citation.volume 27 -
dc.citation.number 8 -
dc.contributor.alternativeName Amarin -
dc.contributor.alternativeName 이수진 -
dc.contributor.alternativeName 이영득 -
dc.contributor.alternativeName 문송 -
dc.contributor.alternativeName 조은영 -
dc.contributor.alternativeName 김태호 -
dc.contributor.alternativeName 강도형 -
dc.contributor.alternativeName 허수진 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.27, no.8, pp.1441 - 1448 -
dc.identifier.doi 10.4014/jmb.1703.03012 -
dc.identifier.scopusid 2-s2.0-85028613550 -
dc.identifier.wosid 000410609900008 -
dc.type.docType Article -
dc.identifier.kciid ART002251876 -
dc.description.journalClass 1 -
dc.subject.keywordPlus SP-NOV. -
dc.subject.keywordPlus MARINE-BACTERIA -
dc.subject.keywordPlus ANTIMICROBIAL ACTIVITY -
dc.subject.keywordPlus BACILLUS-SUBTILIS -
dc.subject.keywordPlus NATURAL-PRODUCTS -
dc.subject.keywordPlus CAPACITY -
dc.subject.keywordPlus CEREUS -
dc.subject.keywordAuthor Colony picking -
dc.subject.keywordAuthor colony scraping -
dc.subject.keywordAuthor broth culture -
dc.subject.keywordAuthor screening -
dc.subject.keywordAuthor antibacterial compounds -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalWebOfScienceCategory Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Microbiology -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 1. Journal Articles
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
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