A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria SCIE SCOPUS KCI

Cited 5 time in WEB OF SCIENCE Cited 7 time in Scopus
Title
A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria
Author(s)
Hettiarachchi, Sachithra Amarin; Lee, Su-Jin; Lee, Youngdeuk; Kwon, Young-Kyung; De Zoysa, Mahanama; Moon, Song; Jo, Eunyoung; Kim, Taeho; Kang, Do-Hyung; Heo, Soo-Jin; Oh, Chulhong
KIOST Author(s)
Lee, Young Deuk(이영득)Jo, Eunyoung(조은영)Kim, Tae Ho(김태호)Kang, Do Hyung(강도형)Heo, Soo Jin(허수진)Oh, Chul Hong(오철홍)
Alternative Author(s)
Hettiarachchi Sachithra Amarin; 이수진; 이영득; 문송; 조은영; 김태호; 강도형; 허수진; 오철홍
Publication Year
2017-08
Abstract
Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2120
DOI
10.4014/jmb.1703.03012
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.27, no.8, pp.1441 - 1448, 2017
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
SP-NOV.; MARINE-BACTERIA; ANTIMICROBIAL ACTIVITY; BACILLUS-SUBTILIS; NATURAL-PRODUCTS; CAPACITY; CEREUS
Keywords
Colony picking; colony scraping; broth culture; screening; antibacterial compounds
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
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