Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra SCIE SCOPUS

DC Field Value Language
dc.contributor.author Park, Mirye -
dc.contributor.author Park, So Yun -
dc.contributor.author Hwang, Jinik -
dc.contributor.author Jung, Seung Won -
dc.contributor.author Lee, Juyun -
dc.contributor.author Chang, Man -
dc.contributor.author Lee, Taek-Kyun -
dc.date.accessioned 2020-04-20T02:25:11Z -
dc.date.available 2020-04-20T02:25:11Z -
dc.date.created 2020-01-28 -
dc.date.issued 2018-05 -
dc.identifier.issn 0253-505X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2108 -
dc.description.abstract Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5 x 10(4) cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0 x 10(4) cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject RED TIDE -
dc.subject DINOFLAGELLATE -
dc.subject MICROALGAE -
dc.subject BLOOM -
dc.subject WATERS -
dc.subject QUANTIFICATION -
dc.subject DINOPHYCEAE -
dc.subject PROBES -
dc.subject LIGHT -
dc.subject RNA -
dc.title Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra -
dc.type Article -
dc.citation.endPage 112 -
dc.citation.startPage 107 -
dc.citation.title ACTA OCEANOLOGICA SINICA -
dc.citation.volume 37 -
dc.citation.number 5 -
dc.contributor.alternativeName 박소윤 -
dc.contributor.alternativeName 정승원 -
dc.contributor.alternativeName 이택견 -
dc.identifier.bibliographicCitation ACTA OCEANOLOGICA SINICA, v.37, no.5, pp.107 - 112 -
dc.identifier.doi 10.1007/s13131-018-1167-7 -
dc.identifier.scopusid 2-s2.0-85046631648 -
dc.identifier.wosid 000431898900013 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus RED TIDE -
dc.subject.keywordPlus DINOFLAGELLATE -
dc.subject.keywordPlus MICROALGAE -
dc.subject.keywordPlus BLOOM -
dc.subject.keywordPlus WATERS -
dc.subject.keywordPlus QUANTIFICATION -
dc.subject.keywordPlus DINOPHYCEAE -
dc.subject.keywordPlus PROBES -
dc.subject.keywordPlus LIGHT -
dc.subject.keywordPlus RNA -
dc.subject.keywordAuthor nuclease protection assay sandwich hybridization -
dc.subject.keywordAuthor Heterocapsa triquetra -
dc.subject.keywordAuthor red tide -
dc.subject.keywordAuthor monitoring -
dc.relation.journalWebOfScienceCategory Oceanography -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Oceanography -
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South Sea Research Institute > Library of Marine Samples > 1. Journal Articles
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