Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra
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Title
- Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra
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Author(s)
- Park, Mirye; Park, So Yun; Hwang, Jinik; Jung, Seung Won; Lee, Juyun; Chang, Man; Lee, Taek-Kyun
- KIOST Author(s)
- Jung, Seung Won(정승원); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 박소윤; 정승원; 이택견
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Publication Year
- 2018-05
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Abstract
- Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5 x 10(4) cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0 x 10(4) cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.
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ISSN
- 0253-505X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2108
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DOI
- 10.1007/s13131-018-1167-7
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Bibliographic Citation
- ACTA OCEANOLOGICA SINICA, v.37, no.5, pp.107 - 112, 2018
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Publisher
- SPRINGER
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Subject
- RED TIDE; DINOFLAGELLATE; MICROALGAE; BLOOM; WATERS; QUANTIFICATION; DINOPHYCEAE; PROBES; LIGHT; RNA
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Keywords
- nuclease protection assay sandwich hybridization; Heterocapsa triquetra; red tide; monitoring
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Type
- Article
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Language
- English
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Document Type
- Article
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