Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Integration of the nuclease protection assay with sandwich hybridization (NPA-SH) for sensitive detection of Heterocapsa triquetra
Author(s)
Park, Mirye; Park, So Yun; Hwang, Jinik; Jung, Seung Won; Lee, Juyun; Chang, Man; Lee, Taek-Kyun
KIOST Author(s)
Jung, Seung Won(정승원)Lee, Taek Kyun(이택견)
Publication Year
2018-05
Abstract
Microalgae are photosynthetic microorganisms that function as primary producers in aquatic ecosystems. Some species of microalgae undergo rapid growth and cause harmful blooms in marine ecosystems. Heterocapsa triquetra is one of the most common bloom-forming species in estuarine and coastal waters worldwide. Although this species does not produce toxins, unlike some other Heterocapsa species, the high density of its blooms can cause significant ecological damage. We developed a H. triquetra species-specific nuclease protection assay sandwich hybridization (NPA-SH) probe that targets the large subunit of ribosomal RNA (LSU rRNA). We tested probe specificity and sensitivity with five other dinoflagellates that also cause red tides. Our assay detected H. triquetra at a concentration of 1.5 x 10(4) cells/mL, more sensitive than required for a red-tide guidance warning by the Korea Ministry of Oceans and Fisheries in 2015 (3.0 x 10(4) cells/mL). We also used the NPA-SH assay to monitor H. triquetra in the Tongyeong region of the southern sea area of Korea during 2014. This method could detect H. triquetra cells within 3 h. Our assay is useful for monitoring H. triquetra under field conditions.
ISSN
0253-505X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2108
DOI
10.1007/s13131-018-1167-7
Bibliographic Citation
ACTA OCEANOLOGICA SINICA, v.37, no.5, pp.107 - 112, 2018
Publisher
SPRINGER
Subject
RED TIDE; DINOFLAGELLATE; MICROALGAE; BLOOM; WATERS; QUANTIFICATION; DINOPHYCEAE; PROBES; LIGHT; RNA
Keywords
nuclease protection assay sandwich hybridization; Heterocapsa triquetra; red tide; monitoring
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Phytoplatnkon taxonomy,Phytoplatnkon physiology,Phycosphere,식물플랑크톤 분류,식물플랑크톤 생리,식물플랑크톤, 바이러스, 박테리아 관계

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