에탄올 생산을 위한 효모 Pichia stipitis의 고정화 KCI

DC Field Value Language
dc.contributor.author 이상은 -
dc.contributor.author 이지은 -
dc.contributor.author 김은진 -
dc.contributor.author 최준호 -
dc.contributor.author 최운용 -
dc.contributor.author 강도형 -
dc.contributor.author 이현용 -
dc.contributor.author 정경환 -
dc.date.accessioned 2020-04-19T23:41:13Z -
dc.date.available 2020-04-19T23:41:13Z -
dc.date.created 2020-01-28 -
dc.date.issued 2012-04 -
dc.identifier.issn 1225-9918 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/1743 -
dc.description.abstract In this study, DEAE-cotton [derivatized by 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl)] was prepared as a carrier for immobilized Pichia stipitis for ethanol production. When cotton was derivatized with 0.5 M DEAE HCl, the yeast cell suspension was adsorbed at 100% of the initial cell OD600 . The adsorbed yeast cells were estimated to be 101.8 mg-dry cells/g-DEAE-cotton. In particular, when a flask culture using the immobilized yeast cells was conducted in a glucose and xylose-containing medium, the yeast cells on the DEAE-cotton gradually produced ethanol, according to glucose and xylose consumption; the ethanol yield was approximately 0.33 g-ethanol/g-monosaccharide. Because DEAE-cotton was successfully used as a carrier for ethanol production from a glucose and xylose-containing medium, we expect that this bioethanol production process may be used for the bioethanol production process from the hydrolysate of lignocellulosic biomass. All the results of DEAE-cotton were compared with those of DEAE-cellulose as a carrier for immobilization. Cotton을 효모 세포( Pichia stipitis )의 고정화 담체로 사용하기 위하여 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl)로 derivatization 시켰다. 0.5 M DEAE HCl로 처리하였을 때, 효모 세포가 완전히 흡착하였으며, 이것은 DEAE-cotton g 당 101.8 mg의 효모 세포가 흡착하는 것이고, DEAE-cellulose에 효모 세포가 흡착하는 양의 약 6배 이상인 것으로 확인되었다. DEAE-cotton을 이용하여 효모 세포를 고정화하고, 이것을 ethanol 생산에 이용하였을 경우, glucose와 xylose가 포함된 배지에서 단당류에 대한 ethanol 수율로 0.33 정도로 ethanol을 생산 할 수 있다는 것을 실험적으로 확인하였다. 이를 이용하여 lignocellulosic bomass의 가수분해물로부터 bioethanol 생산에 이용될 수 있을 것으로 기대되어진다. DEAE-cotton에서 얻어진 결과는 DEAE-cellulose에서 같은 실험을 실시하여 서로 비교 분석하였다. -
dc.description.uri 2 -
dc.language Korean -
dc.publisher 한국생명과학회 -
dc.title 에탄올 생산을 위한 효모 Pichia stipitis의 고정화 -
dc.title.alternative Immobilization of Yeast Pichia stipitis for Ethanol Production -
dc.type Article -
dc.citation.endPage 515 -
dc.citation.startPage 508 -
dc.citation.title 생명과학회지 -
dc.citation.volume 22 -
dc.citation.number 4 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation 생명과학회지, v.22, no.4, pp.508 - 515 -
dc.identifier.doi 10.5352/JLS.2012.22.4.508 -
dc.identifier.kciid ART001660283 -
dc.description.journalClass 2 -
dc.description.isOpenAccess N -
dc.subject.keywordAuthor Cotton -
dc.subject.keywordAuthor DEAE·HCl derivatization -
dc.subject.keywordAuthor yeast cell immobilization -
dc.subject.keywordAuthor ethanol production -
dc.subject.keywordAuthor Pichia stipitis -
dc.description.journalRegisteredClass kci -
Appears in Collections:
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
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