에탄올 생산을 위한 효모 Pichia stipitis의 고정화 KCI
DC Field | Value | Language |
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dc.contributor.author | 이상은 | - |
dc.contributor.author | 이지은 | - |
dc.contributor.author | 김은진 | - |
dc.contributor.author | 최준호 | - |
dc.contributor.author | 최운용 | - |
dc.contributor.author | 강도형 | - |
dc.contributor.author | 이현용 | - |
dc.contributor.author | 정경환 | - |
dc.date.accessioned | 2020-04-19T23:41:13Z | - |
dc.date.available | 2020-04-19T23:41:13Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.issn | 1225-9918 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/1743 | - |
dc.description.abstract | In this study, DEAE-cotton [derivatized by 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl)] was prepared as a carrier for immobilized Pichia stipitis for ethanol production. When cotton was derivatized with 0.5 M DEAE HCl, the yeast cell suspension was adsorbed at 100% of the initial cell OD600 . The adsorbed yeast cells were estimated to be 101.8 mg-dry cells/g-DEAE-cotton. In particular, when a flask culture using the immobilized yeast cells was conducted in a glucose and xylose-containing medium, the yeast cells on the DEAE-cotton gradually produced ethanol, according to glucose and xylose consumption; the ethanol yield was approximately 0.33 g-ethanol/g-monosaccharide. Because DEAE-cotton was successfully used as a carrier for ethanol production from a glucose and xylose-containing medium, we expect that this bioethanol production process may be used for the bioethanol production process from the hydrolysate of lignocellulosic biomass. All the results of DEAE-cotton were compared with those of DEAE-cellulose as a carrier for immobilization. Cotton을 효모 세포( Pichia stipitis )의 고정화 담체로 사용하기 위하여 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl)로 derivatization 시켰다. 0.5 M DEAE HCl로 처리하였을 때, 효모 세포가 완전히 흡착하였으며, 이것은 DEAE-cotton g 당 101.8 mg의 효모 세포가 흡착하는 것이고, DEAE-cellulose에 효모 세포가 흡착하는 양의 약 6배 이상인 것으로 확인되었다. DEAE-cotton을 이용하여 효모 세포를 고정화하고, 이것을 ethanol 생산에 이용하였을 경우, glucose와 xylose가 포함된 배지에서 단당류에 대한 ethanol 수율로 0.33 정도로 ethanol을 생산 할 수 있다는 것을 실험적으로 확인하였다. 이를 이용하여 lignocellulosic bomass의 가수분해물로부터 bioethanol 생산에 이용될 수 있을 것으로 기대되어진다. DEAE-cotton에서 얻어진 결과는 DEAE-cellulose에서 같은 실험을 실시하여 서로 비교 분석하였다. | - |
dc.description.uri | 2 | - |
dc.language | Korean | - |
dc.publisher | 한국생명과학회 | - |
dc.title | 에탄올 생산을 위한 효모 Pichia stipitis의 고정화 | - |
dc.title.alternative | Immobilization of Yeast Pichia stipitis for Ethanol Production | - |
dc.type | Article | - |
dc.citation.endPage | 515 | - |
dc.citation.startPage | 508 | - |
dc.citation.title | 생명과학회지 | - |
dc.citation.volume | 22 | - |
dc.citation.number | 4 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | 생명과학회지, v.22, no.4, pp.508 - 515 | - |
dc.identifier.doi | 10.5352/JLS.2012.22.4.508 | - |
dc.identifier.kciid | ART001660283 | - |
dc.description.journalClass | 2 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Cotton | - |
dc.subject.keywordAuthor | DEAE·HCl derivatization | - |
dc.subject.keywordAuthor | yeast cell immobilization | - |
dc.subject.keywordAuthor | ethanol production | - |
dc.subject.keywordAuthor | Pichia stipitis | - |
dc.description.journalRegisteredClass | kci | - |