Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge KCI

Title
Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge
Alternative Title
Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge
Author(s)
Udeni Menike; Krishan Ariyasiri; Jin-young Choi; 이영득; W.D.N.Wickramaarachchi; H.K.A. Premachandra; Jehee Lee; Mahanama De Zoysa
KIOST Author(s)
Lee, Youngdeuk(이영득)
Publication Year
2013-06
Abstract
Cathepsins are lysosomal/cysteine proteases belong to papain family (C1 family) that is involved in intracellular protein degradation, antigen processing, hormone maturation, and immune responses. In this study, member of cathepsin family was identified from Manila clam (Mc-Cathepsin D) and investigated the immune response against brown ring disease (BRD) causing Vibrio tapetis challenge. The identified Mc-Cathepsin D gene encodes characteristic features typical for the cathepsin family including eukaryotic and viral aspartyl protease signature domain and two highly conserved active sites ( 84VVFDTGSSNLWV95 and 270IADTGTSLLAG281 ). Moreover, MC-Cathepsin D shows higher identity values (-50-70%) and conserved amino acids with known cathepsin D members. Transcriptional results (by quantitative real-time RT-PCR) showed that Mc-Cathepsin D was expressed at higher levels in gills and hemocytes than mantle, adductor muscle, foot, and siphon. After the V. tapetis challenge under laboratory conditions, Mc-Cathepsin D mRNA was up-regulated in gills and hemocytes. Present study indicates that Mc-Cathepsin D is constitutively expressed in different tissues and potentially inducible when infecting BRD by V. tapetis. It is further suggesting that Mc-Cathepsin D may be involved in multiple role including immune response reactions against BRD.
ISSN
1225-3480
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1674
Bibliographic Citation
The Korean Journal of Malacology, v.29, no.2, pp.155 - 161, 2013
Publisher
한국패류학회
Keywords
Brown ring disease; Manila clam (Ruditapes philipphinarum); Cathepsin D; V. tapetis.
Type
Article
Language
English
Publisher
한국패류학회
Related Researcher
Research Interests

Marine Biotechnology,Marine Organism Molecular genetics,Marine Bacterial engineering,해양생물공학,해양생물분자유전학,해양미생물공학

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