초음파 추출법에 의한 감태 추출물의 페놀성 화합물 함량, DPPH 라디칼 소거 활성 및 tyrosinase 저해 활성 분석 KCI

Title
초음파 추출법에 의한 감태 추출물의 페놀성 화합물 함량, DPPH 라디칼 소거 활성 및 tyrosinase 저해 활성 분석
Alternative Title
Phenolic Content, DPPH Radical Scavenging, and Tyrosinase Inhibitory Activities of Ecklonia cava Extracted with the Ultrasonic Wave Method
Author(s)
So Jung Kim; 김동균; 박종범; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2013-07
Abstract
The applicability of the ultrasonic wave method to the extraction of useful components from seaweeds was investigated. Extracts from freeze-dried Ecklonia cava powder were prepared with hot water ( 65∘C ), water ( 24∘C ), 50% ethanol, and 100% ethanol, and ultrasonic extraction was also performed. The content of phenolic compounds and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and tyrosinase inhibitory activity of the extracts were analyzed, and differences in the data obtained by the ultrasonic extraction and the traditional extraction methods were compared. The phenolic content in the E. cava extract by ultrasonic extraction (142.80 mg/g) was approximately 14 times higher than the phenolic content in the hot water extract (10.03 mg/g). The DPPH radical scavenging and the tyrosinase inhibitory activities of the ultrasonic extract were approximately 4 times and 14 times higher than the hot water extracts, respectively. The correlation between the phenolic content and the DPPH radical scavenging activity ( R2 =99.4 99.47) and between the phenolic content and the tyrosinase inhibitory activity ( R2 =99.9 99.99) was very high. These results indicate that ultrasonic extraction is more suitable than traditional extraction for the extraction of useful components from E. cava.

해조류 유래 유용성분 추출을 위한 초음파 추출법의 적용성을 검토하였다. 동결건조한 감태 분말로부터 열수( 65∘C ), 물( 24∘C ), 50% 에탄올 및 100% 에탄올 뿐만 아니라 초음파 추출을 수행하여, 추출물을 제조하였다. 추출물의 페놀성 화합물 함량, DPPH 라디칼 소거 활성 및 tyrosinase 저해 활성을 분석하고, 초음파 추출과 전통적인 추출법의 차이를 비교하였다. 초음파 추출에 의한 감태 추출물의 페놀성 화합물의 함량(142.80 mg/g)은 열수 추출물의 페놀성 화합물 함량(10.03 mg/g)의 약 14배 이상 높았다. 열수 추출물에 비해 초음파 추출물의 DPPH 라디칼 소거 활성은 약 4배, tyrosinase 저해 활성은 약 14배 이상 높은 것으로 나타났다. 특히 초음파 추출법에 의한 추출물에서의 페놀성 화합물 함량과 DPPH 라디칼 소거활성 및 페놀성 화합물 함량과 tyrosinase 저해 활성간의 상관관계가 매우 높은 것(각각 R2 =99.4 99.47 및 99.99)으로 나타났다. 이상의 결과는 전통적인 용매 추출법보다 초음파 추출법이 감태의 유용성분 추출에 더 적합함을 의미한다.
ISSN
1225-9918
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1667
DOI
10.5352/JLS.2013.23.7.913
Bibliographic Citation
생명과학회지, v.23, no.7, pp.913 - 918, 2013
Publisher
한국생명과학회
Keywords
Ultrasonic extraction; Ecklonia cava; phenolics; DPPH assay; tyrosinase inhibitory activity
Type
Article
Language
Korean
Publisher
한국생명과학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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