Identification and quantification of microplastics using Nile Red staining SCIE SCOPUS

Cited 123 time in WEB OF SCIENCE Cited 131 time in Scopus
Title
Identification and quantification of microplastics using Nile Red staining
Author(s)
Shim, Won Joon; Song, Young Kyoung; Hong, Sang Hee; Jang, Mi
KIOST Author(s)
Shim, Won Joon(심원준)Song, Young Kyoung(송영경)Hong, Sang Hee(홍상희)Jang, Mi(장미)
Publication Year
2016-12
Abstract
We investigated the applicability of Nile Red (NR), a fluorescent dye, for microplastic analysis, and determined the optimal staining conditions. Five mg/L NR solution in n-hexane effectively stained plastics, and they were easily recognized in green fluorescence. The NR staining method was successfully applied to micro-sized polyethylene, polypropylene, polystyrene, polycarbonate, polyurethane, and poly(ethylene-vinyl acetate), except for polyvinylchloride, polyamide and polyester. The recovery rate of polyethylene (100-300 pm) spiked to pretreated natural sand was 98% in the NR stating method, which was not significantly (p < 0.05) different with FT-IR identification. The NR staining method was suitable for discriminating fragmented polypropylene particles from large numbers of sand particles in laboratory weathering test samples. The method is straightforward and quick for identifying and quantifying polymer particles in the laboratory controlled samples. Further studies, however, are necessary to investigate the application of NR staining to field samples with organic remnants. (C) 2016 Elsevier Ltd. All rights reserved.
ISSN
0025-326X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1374
DOI
10.1016/j.marpolbul.2016.10.049
Bibliographic Citation
MARINE POLLUTION BULLETIN, v.113, no.1-2, pp.469 - 476, 2016
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Nile Red; staining; identification; microplastics
Type
Article
Language
English
Document Type
Article
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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