Statistical optimization of medium for formate-driven bio-hydrogen production by the hyperthermophilic archaeon, thermococus onnurineus SCOPUS KCI

Title
Statistical optimization of medium for formate-driven bio-hydrogen production by the hyperthermophilic archaeon, thermococus onnurineus
Author(s)
Lee, S.-M.; Kim, T.W.; Lee, H.S.; Lee, J.-H.; Kang, S.G.
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2017
Abstract
Medium compositions for the hyperthermophilic archaeon, Thermococcus onnurineus NA1 was statistically optimized to enhance formate-driven hydrogen (H 2 ) production by using response surface methodology. From the Plackett-Burman design-based experiment, it was confirmed that among the minor components of medium such as KCl, MgSO 4 , NH 4 Cl, Cystein-HCl, trace elements, Fe-EDTA and CaCl 2 , the trace elements were screened as the only positively effective components with respect to H 2 production. Subsequently, the optimal concentrations of the trace elements and the major components of a medium such as NaCl, yeast extract and sodium formate were determined from the five-level central composite design (CCD)-based experiment. The resulting quadratic model predicted the maximum H 2 production of 46.6 mmol/L in serum bottle and it was validated experimentally using the optimal medium initially supplemented with 26.70 g/L of NaCl, 9.81 g/L of sodium formate, 3.50 g/L of yeast extract and 4.59 mL/L of trace elements. From the duplicate batch cultivations in the fermentor using the optimized medium, the a maximum H 2 production rate up to 71.8 mmol/L/h could be obtained, which was a 65% enhanced value compared with that obtained using the control medium, showing the high efficiency of the optimized medium. © 2018, Korea Ocean Research and Development Institute. All rights reserved.
ISSN
1598-141X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1316
DOI
10.4217/OPR.2017.39.4.269
Bibliographic Citation
Ocean and Polar Research, v.39, no.4, pp.269 - 277, 2017
Publisher
Korea Ocean Research and Development Institute
Subject
microorganism; optimization; statistical analysis; trace element; Archaea; Thermococcus
Keywords
Bio-hydrogen production; Formate; Hyperthermophilic archaeon; Response surface methodology; Thermococcus onnurineus NA1
Type
Article
Language
Korean
Document Type
Article
Publisher
Korea Ocean Research and Development Institute
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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