Echinicola strongylocentroti sp nov., isolated from a sea urchin Strongylocentrotus intermedius

Abstract

A yellowish-orange-pigmented marine bacterium, designated MEBiC08714(inverted perpendicular) was isolated from a sea urchin Strongylocentrotus intermedius collected at the west edge of the East Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that MEBiC08714(inverted perpendicular) was affiliated with the genus Echinicola and that the strain was most closely related to Echinicola vietnamensis KCTC 12713(inverted perpendicular) (96.9 %), followed by 'Echinicola shivajiensis' JCM 17847 (96.3 %), Echinicola jeungdonensis KCTC 23122(inverted perpendicular) (96.1 %), and Echinicola pacifica KCTC 12368(inverted perpendicular) (95.0 %). Cells were strictly aerobic, Gram-stain-negative, rod shaped, flexirubin-type pigments-negative and motile by gliding. Growth was observed at 20-35 degrees C (optimum 25 degrees C), at pH 6-11 (optimum pH 7.0), and with 0-13% NaCl (optimum 2 %). This strain was able to hydrolyze agar and starch. The polar lipids of MEBiC08714(inverted perpendicular) contained phosphatidylethanolamine, an unidentified phospholipid, and four unidentified lipids. The major fatty acids were iso-C-15 : 0 (27.5 %), iso-C-17 : 0 3-OH (11.5 %), anteiso-C-15 : 0 (5.2 %), summed feature 3 (C-16: 1 omega 7c and/ or C-16: 1 omega 6c, 20.3 %) and summed feature 9 (iso-C-17 : 1 omega 9c and/or 10-methyl C-16 : 06, 6.3 %). The DNA G+C content was 44.2 mol% and the major respiratory quinone was MK-7. On the basis of these polyphasic taxonomic data, MEBiC08714(inverted perpendicular) represents a novel species of the genus Echinicola, for which the name Echinicola strongylocentroti sp. nov. is proposed. The type strain is MEBiC08714(inverted perpendicular) (= KCTC 52052(inverted perpendicular) = JCM 31307(inverted perpendicular)).