Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity SCIE SCOPUS

DC Field Value Language
dc.contributor.author Jo, Eunyoung -
dc.contributor.author Elvitigala, Don Anushka Sandaruwan -
dc.contributor.author Wan, Qiang -
dc.contributor.author Oh, Minyoung -
dc.contributor.author Oh, Chulhong -
dc.contributor.author Lee, Jehee -
dc.date.accessioned 2020-04-16T09:55:06Z -
dc.date.available 2020-04-16T09:55:06Z -
dc.date.created 2020-01-28 -
dc.date.issued 2017-12 -
dc.identifier.issn 0145-305X -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/1105 -
dc.description.abstract Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippo campus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52.6 kDa and a theoretical isoelectric point of 8.26. The deduced amino acid sequence contains a single carbohydrate recognition domain (CRD), in which six conserved cysteine residues and two Ca2+-binding site motifs (QPN, WND) were identified. Based on pairwise sequence analysis, ShDCS-like exhibits the highest amino acid identity (94.6%) and similarity (97.4%) with DC-SIGN-like counterpart from tiger tail seahorse Hippocampus comes. Quantitative real-time PCR revealed that ShDCS-like mRNA is transcribed universally in all tissues examined, but with abundance in kidney and gill tissues. The basal mRNA expression of ShDCS-like was modulated in blood cell, kidney, gill and liver tissues in response to the stimulation of healthy fish with lipopolysaccharides (LPS), Edwardsiella tarda, or Streptococcus iniae. Moreover, recombinant ShDCS-like-CRD domain exhibited detectable agglutination activity against different bacteria. Collectively, these results suggest that ShDCS-like may potentially involve in immune function in big belly seahorses. (C) 2017 Elsevier Ltd. All rights reserved. -
dc.description.uri 1 -
dc.language English -
dc.publisher ELSEVIER SCI LTD -
dc.subject C-TYPE LECTIN -
dc.subject DENDRITIC CELL TRAFFICKING -
dc.subject VIBRIO-ALGINOLYTICUS -
dc.subject PROCAMBARUS-CLARKII -
dc.subject ERIOCHEIR-SINENSIS -
dc.subject INNATE IMMUNITY -
dc.subject ANIMAL LECTINS -
dc.subject TELEOST FISH -
dc.subject RECOGNITION -
dc.subject EXPRESSION -
dc.title Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity -
dc.type Article -
dc.citation.endPage 279 -
dc.citation.startPage 270 -
dc.citation.title DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY -
dc.citation.volume 77 -
dc.contributor.alternativeName 조은영 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.77, pp.270 - 279 -
dc.identifier.doi 10.1016/j.dci.2017.08.017 -
dc.identifier.scopusid 2-s2.0-85028763462 -
dc.identifier.wosid 000413387100029 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus C-TYPE LECTIN -
dc.subject.keywordPlus DENDRITIC CELL TRAFFICKING -
dc.subject.keywordPlus VIBRIO-ALGINOLYTICUS -
dc.subject.keywordPlus PROCAMBARUS-CLARKII -
dc.subject.keywordPlus ERIOCHEIR-SINENSIS -
dc.subject.keywordPlus INNATE IMMUNITY -
dc.subject.keywordPlus ANIMAL LECTINS -
dc.subject.keywordPlus TELEOST FISH -
dc.subject.keywordPlus RECOGNITION -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordAuthor Bacterial agglutination activity -
dc.subject.keywordAuthor Big belly seahorse -
dc.subject.keywordAuthor DC-SIGN -
dc.subject.keywordAuthor Immune stimulation -
dc.subject.keywordAuthor Transcription analysis -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.relation.journalWebOfScienceCategory Immunology -
dc.relation.journalWebOfScienceCategory Zoology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Fisheries -
dc.relation.journalResearchArea Immunology -
dc.relation.journalResearchArea Zoology -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
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