Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Eunyoung | - |
dc.contributor.author | Elvitigala, Don Anushka Sandaruwan | - |
dc.contributor.author | Wan, Qiang | - |
dc.contributor.author | Oh, Minyoung | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-16T09:55:06Z | - |
dc.date.available | 2020-04-16T09:55:06Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/1105 | - |
dc.description.abstract | Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippo campus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52.6 kDa and a theoretical isoelectric point of 8.26. The deduced amino acid sequence contains a single carbohydrate recognition domain (CRD), in which six conserved cysteine residues and two Ca2+-binding site motifs (QPN, WND) were identified. Based on pairwise sequence analysis, ShDCS-like exhibits the highest amino acid identity (94.6%) and similarity (97.4%) with DC-SIGN-like counterpart from tiger tail seahorse Hippocampus comes. Quantitative real-time PCR revealed that ShDCS-like mRNA is transcribed universally in all tissues examined, but with abundance in kidney and gill tissues. The basal mRNA expression of ShDCS-like was modulated in blood cell, kidney, gill and liver tissues in response to the stimulation of healthy fish with lipopolysaccharides (LPS), Edwardsiella tarda, or Streptococcus iniae. Moreover, recombinant ShDCS-like-CRD domain exhibited detectable agglutination activity against different bacteria. Collectively, these results suggest that ShDCS-like may potentially involve in immune function in big belly seahorses. (C) 2017 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | C-TYPE LECTIN | - |
dc.subject | DENDRITIC CELL TRAFFICKING | - |
dc.subject | VIBRIO-ALGINOLYTICUS | - |
dc.subject | PROCAMBARUS-CLARKII | - |
dc.subject | ERIOCHEIR-SINENSIS | - |
dc.subject | INNATE IMMUNITY | - |
dc.subject | ANIMAL LECTINS | - |
dc.subject | TELEOST FISH | - |
dc.subject | RECOGNITION | - |
dc.subject | EXPRESSION | - |
dc.title | Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity | - |
dc.type | Article | - |
dc.citation.endPage | 279 | - |
dc.citation.startPage | 270 | - |
dc.citation.title | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | - |
dc.citation.volume | 77 | - |
dc.contributor.alternativeName | 조은영 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.identifier.bibliographicCitation | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.77, pp.270 - 279 | - |
dc.identifier.doi | 10.1016/j.dci.2017.08.017 | - |
dc.identifier.scopusid | 2-s2.0-85028763462 | - |
dc.identifier.wosid | 000413387100029 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | C-TYPE LECTIN | - |
dc.subject.keywordPlus | DENDRITIC CELL TRAFFICKING | - |
dc.subject.keywordPlus | VIBRIO-ALGINOLYTICUS | - |
dc.subject.keywordPlus | PROCAMBARUS-CLARKII | - |
dc.subject.keywordPlus | ERIOCHEIR-SINENSIS | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | ANIMAL LECTINS | - |
dc.subject.keywordPlus | TELEOST FISH | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Bacterial agglutination activity | - |
dc.subject.keywordAuthor | Big belly seahorse | - |
dc.subject.keywordAuthor | DC-SIGN | - |
dc.subject.keywordAuthor | Immune stimulation | - |
dc.subject.keywordAuthor | Transcription analysis | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Zoology | - |