Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity
Author(s)
Jo, Eunyoung; Elvitigala, Don Anushka Sandaruwan; Wan, Qiang; Oh, Minyoung; Oh, Chulhong; Lee, Jehee
KIOST Author(s)
Jo, Eunyoung(조은영)Oh, Chulhong(오철홍)
Publication Year
2017-12
Abstract
Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippo campus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52.6 kDa and a theoretical isoelectric point of 8.26. The deduced amino acid sequence contains a single carbohydrate recognition domain (CRD), in which six conserved cysteine residues and two Ca2+-binding site motifs (QPN, WND) were identified. Based on pairwise sequence analysis, ShDCS-like exhibits the highest amino acid identity (94.6%) and similarity (97.4%) with DC-SIGN-like counterpart from tiger tail seahorse Hippocampus comes. Quantitative real-time PCR revealed that ShDCS-like mRNA is transcribed universally in all tissues examined, but with abundance in kidney and gill tissues. The basal mRNA expression of ShDCS-like was modulated in blood cell, kidney, gill and liver tissues in response to the stimulation of healthy fish with lipopolysaccharides (LPS), Edwardsiella tarda, or Streptococcus iniae. Moreover, recombinant ShDCS-like-CRD domain exhibited detectable agglutination activity against different bacteria. Collectively, these results suggest that ShDCS-like may potentially involve in immune function in big belly seahorses. (C) 2017 Elsevier Ltd. All rights reserved.
ISSN
0145-305X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1105
DOI
10.1016/j.dci.2017.08.017
Bibliographic Citation
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.77, pp.270 - 279, 2017
Publisher
ELSEVIER SCI LTD
Subject
C-TYPE LECTIN; DENDRITIC CELL TRAFFICKING; VIBRIO-ALGINOLYTICUS; PROCAMBARUS-CLARKII; ERIOCHEIR-SINENSIS; INNATE IMMUNITY; ANIMAL LECTINS; TELEOST FISH; RECOGNITION; EXPRESSION
Keywords
Big belly seahorse; DC-SIGN; Immune stimulation; Transcription analysis; Bacterial agglutination activity
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Related Researcher
Research Interests

Marine Life Science,해양생명과학

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