Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity
SCIE
SCOPUS
Cited 3 time in
WEB OF SCIENCE
Cited 3 time in
Scopus
-
Title
- Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity
-
Author(s)
- Jo, Eunyoung; Elvitigala, Don Anushka Sandaruwan; Wan, Qiang; Oh, Minyoung; Oh, Chulhong; Lee, Jehee
- KIOST Author(s)
- Jo, Eunyoung(조은영); Oh, Chul Hong(오철홍)
-
Publication Year
- 2017-12
-
Abstract
- Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippo campus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52.6 kDa and a theoretical isoelectric point of 8.26. The deduced amino acid sequence contains a single carbohydrate recognition domain (CRD), in which six conserved cysteine residues and two Ca2+-binding site motifs (QPN, WND) were identified. Based on pairwise sequence analysis, ShDCS-like exhibits the highest amino acid identity (94.6%) and similarity (97.4%) with DC-SIGN-like counterpart from tiger tail seahorse Hippocampus comes. Quantitative real-time PCR revealed that ShDCS-like mRNA is transcribed universally in all tissues examined, but with abundance in kidney and gill tissues. The basal mRNA expression of ShDCS-like was modulated in blood cell, kidney, gill and liver tissues in response to the stimulation of healthy fish with lipopolysaccharides (LPS), Edwardsiella tarda, or Streptococcus iniae. Moreover, recombinant ShDCS-like-CRD domain exhibited detectable agglutination activity against different bacteria. Collectively, these results suggest that ShDCS-like may potentially involve in immune function in big belly seahorses. (C) 2017 Elsevier Ltd. All rights reserved.
-
ISSN
- 0145-305X
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/1105
-
DOI
- 10.1016/j.dci.2017.08.017
-
Bibliographic Citation
- DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.77, pp.270 - 279, 2017
-
Publisher
- ELSEVIER SCI LTD
-
Subject
- C-TYPE LECTIN; DENDRITIC CELL TRAFFICKING; VIBRIO-ALGINOLYTICUS; PROCAMBARUS-CLARKII; ERIOCHEIR-SINENSIS; INNATE IMMUNITY; ANIMAL LECTINS; TELEOST FISH; RECOGNITION; EXPRESSION
-
Keywords
- Big belly seahorse; DC-SIGN; Immune stimulation; Transcription analysis; Bacterial agglutination activity
-
Type
- Article
-
Language
- English
-
Document Type
- Article
-
Publisher
- ELSEVIER SCI LTD
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.