Anti-inflammation and Anti-Cancer Activity of Ethanol Extract of Antarctic Freshwater Microalga, Micractinium sp. SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 11 time in Scopus
Title
Anti-inflammation and Anti-Cancer Activity of Ethanol Extract of Antarctic Freshwater Microalga, Micractinium sp.
Author(s)
Suh, Sung-Suk; Hong, Ju-Mi; Kim, Eun Jae; Jung, Seung Won; Kim, Sun-Mi; Kim, Jung Eun; Kim, Il-Chan; Kim, Sanghee
KIOST Author(s)
Jung, Seung Won(정승원)
Publication Year
2018
Abstract
Inflammation mediated by the innate immune system is an organism's protective mechanism against infectious environmental risk factors. It is also a driver of the pathogeneses of various human diseases, including cancer development and progression. Microalgae are increasingly being focused on as sources of bioactive molecules with therapeutic potential against various diseases. Furthermore, the antioxidant, anti-inflammatory, and anticancer potentials of microalgae and their secondary metabolites have been widely reported. However, the underlying mechanisms remain to be elucidated. Therefore, in this study, we investigated the molecular mechanisms underlying the anti-inflammatory and anticancer activities of the ethanol extract of the Antarctic freshwater microalga Micractinium sp. (ETMI) by several in vitro assays using RAW 264.7 macrophages and HCTI 16 human colon cancer cells. ETMI exerted its anti-inflammatory activity by modulating the main inflammatory indicators such as cyclooxygenase (COX)-2, interleukin (IL)-6, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-alpha, and nitric oxide (NO) in a dose-dependent manner. In addition, ETMI exerted cytotoxic activity against HCTI 16 cells in a dose-dependent manner, leading to significantly reduced cancer cell proliferation. Further, it induced cell cycle arrest in the G 1 phase through the regulation of hallmark genes of the G I/S phase transition, including CDKN IA, and cyclin-dependent kinase 4 and 6 (CDK4 and CDK6, respectively). At the transcriptional level, the expression of CDKN I A gradually increased in response to ETMI treatment while that of CDK4 and CDK6 decreased. Taken together, our findings suggest that the anti-inflammatory and anticancer activities of the Antarctic freshwater microalga, Micractinium sp., and ETMI may provide a new clue for understanding the molecular link between inflammation and cancer and that ETMI may be a potential anticancer agent for targeted therapy of colorectal cancer.
ISSN
1449-1907
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1044
DOI
10.7150/ijms.26410
Bibliographic Citation
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, v.15, no.9, pp.929 - 936, 2018
Publisher
IVYSPRING INT PUBL
Subject
NF-KAPPA-B; DISEASE; HOMEOSTASIS; EXPRESSION; CYTOKINES; CANCER
Keywords
Inflammation; cancer; Micractinium sp.; proinflammatory cytokines; HCT116
Type
Article
Language
English
Document Type
Article
Publisher
IVYSPRING INT PUBL
Related Researcher
Research Interests

Phytoplatnkon taxonomy,Phytoplatnkon physiology,Phycosphere,식물플랑크톤 분류,식물플랑크톤 생리,식물플랑크톤, 바이러스, 박테리아 관계

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse