Euzebyella algicola sp nov., a marine bacterium of the family Flavobacteriaceae, isolated from green algae

Abstract

A Gram-negative, yellow-pigmented, non-flagellated, gliding, rod-shaped and aerobic bacterium, designated MEBiC 12267(T), was isolated from green algae of Jeju Island. 16S rRNA gene sequence analysis revealed that the strain MEBiC 12267(T) was affiliated to the genus Euzebyella of the family Flavobacteriaceae and showed the highest similarity to Euzebyella marina KCTC 42440(T) (98.5 %). The DNA-DNA relatedness value of strain MEBiC 12267(T) with E. marina KCTC 42440(T) was 25%. Growth was observed at 10-37 degrees C (optimum, 30-33 degrees C), at pH 6.0-9.5 (optimum, 8.0-8.5) and with 0.5-9.0 % (w/v) NaCl (optimum, 2.5-3.5 %). The predominant cellular fatty acids were iso-C-15 : 0, iso-C-15 : 1 G and iso-C(17 : 0 )3-OH. The major respiratory quinone was MK-6. Polar lipids included phosphatidylethanolamine, seven unidentified lipids and two unidentified aminolipids. The DNA G+C content was 40.7 mol%. On the basis of the data from the polyphasic taxonomic study, it was concluded that the strain MEBiC 12267(T) represents a novel species within the genus Euzebyella, for which the name Euzebyella algicola sp. nov. is proposed. The type strain of E. algicola is MEBiC 12267(T) (=KCCM 43264(T)=JCM 321 70(T)).