Planktonic bivalve larvae identification and quantification in Gomso Bay, South Korea, using next-generation sequencing analysis and microscopic observations SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Planktonic bivalve larvae identification and quantification in Gomso Bay, South Korea, using next-generation sequencing analysis and microscopic observations
Author(s)
Jung, Seung Won; Kim, Hyun Jung; Park, Joon Sang; Lee, Taek-Kyun; Shin, Kyoungsoon; Jeong, Su-Young; Hwang, Jae Youn; Yoo, Jae-Won
KIOST Author(s)
Jung, Seung Won(정승원)Kim, Hyun Jung(김현정)Park, Joon Sang(박준상)Lee, Taek Kyun(이택견)Shin, Kyoung Soon(신경순)
Publication Year
2018-03-01
Abstract
Planktonic larval stages of bivalves are notoriously difficult to identify due to their similar morphology. To evaluate changed patterns in the presented planktonic bivalve larvae within Gomso Bay (Yellow Sea, South Korea), three sites were sampled twice a month from June to October 2015 and were analysed using next-generation sequencing (NGS) and light microscopy. Bivalve larvae revealed different patterns, and at least five bivalve taxa were only detected by the NGS. According to the NGS results, most bivalve larvae were Crassostrea gigas (37%), followed by Ruditapes philippinarum (26%), and Solen strictus (23%); Mactra chinensis and M. veneriformis accounted for< 1% of the bivalve larvae. C. gigas and S. strictus were most abundant in late June, but rare in the other investigated periods, while R. philippinarum was more abundant in June and late September. Microscopic observations showed a similar trend to the NGS in terms of bivalve larvae presence, although they were difficult to identify the exact species. Thus, morphological and molecular approaches should be combined in future studies to provide accurate sequences of correctly identified specimens to develop NGS libraries.
ISSN
0044-8486
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/993
DOI
10.1016/j.aquaculture.2018.02.053
Bibliographic Citation
AQUACULTURE, v.490, pp.297 - 302, 2018
Publisher
ELSEVIER SCIENCE BV
Subject
GROSS BIOCHEMICAL-COMPOSITION; CLAM RUDITAPES-PHILIPPINARUM; BACTERIOPLANKTON COMMUNITIES; TEMPERATURE; SETTLEMENT; DIVERSITY; BARNACLES; PATTERNS; GROWTH; BIAS
Keywords
Bivalve larvae; Next-generation sequencing; Gomso Bay; Planktonic stage; Morphological analysis
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
Related Researcher
Research Interests

Marine biology,Taxonomy,Diatom,해양생물학,생물분류학,규조류

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse