De novo transcriptome assembly of brackish water flea Diaphanosoma celebensis based on short-term cadmium and benzo[a]pyrene exposure experiments SCIE SCOPUS

Cited 19 time in WEB OF SCIENCE Cited 21 time in Scopus
Title
De novo transcriptome assembly of brackish water flea Diaphanosoma celebensis based on short-term cadmium and benzo[a]pyrene exposure experiments
Author(s)
Kim, Bo-Mi; Kang, Seunghyun; Kim, Ryeo-Ok; Jung, Jee-Hyun; Lee, Kyun-Woo; Rhee, Jae-Sung; Lee, Young-Mi
KIOST Author(s)
Jung, Jee Hyun(정지현)Lee, Kyun Woo(이균우)
Alternative Author(s)
정지현; 이균우
Publication Year
2018-11-17
Abstract
To develop a brackish water flea as a promising model for marine monitoring, Diaphanosoma celebensis were exposed to two pollutants, cadmium (Cd) and benzo[a] pyrene (BaP), which have different chemical characteristics and distinct modes of metabolic action on aquatic animals. Twenty-four hours after exposure to Cd (2mg/L) or BaP (25 mu g/L), whole body transcriptomes were analyzed. In total, 99.6 Mbp were assembled from nine libraries, resulting in 98,458 transcripts with an N50 of 1883 bp and an average contig length of 968 bp. Functional gene annotations were performed using Gene Ontology, Eukaryotic Orthologous Groups, and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Cd significantly modulated endocrine and digestive enzyme system. Following BaP treatment, DNA repair and circadian rhythm related metabolisms were significantly modulated. Both the chemicals induced stress response and detoxification metabolism. This brackish water flea genomic information will be useful to monitor estuaries and coastal regions, as water fleas have been confirmed as promising sentinel models in freshwater ecosystems.
ISSN
1601-5223
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/805
DOI
10.1186/s41065-018-0075-3
Bibliographic Citation
HEREDITAS, v.155, 2018
Publisher
BMC
Subject
ANTIOXIDANT ENZYMES; DAPHNIA-MAGNA; HEAVY-METALS; EXPRESSION; GENES; BENZO(A)PYRENE; BIOMARKERS; STINGELIN; PATHWAY; DAMAGE
Keywords
Benzo[a]pyrene; brackish water flea; Cadmium; Diaphanosoma celebensis; Transcriptome
Type
Article
Language
English
Document Type
Article
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