Acclimation of Prorocentrum minimum (Dinophyceae) to prolonged darkness by use of an alternative carbon source from triacylglycerides and galactolipids SCIE SCOPUS

Cited 26 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Acclimation of Prorocentrum minimum (Dinophyceae) to prolonged darkness by use of an alternative carbon source from triacylglycerides and galactolipids
Author(s)
Manoharan, K; Lee, TK; Cha, JM; Kim, JH; Lee, WS; Chang, M; Park, CW; Cho, JH
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
1999-04
Abstract
The dinoflagellate Prorocentrum minimum (Pavillard) Schiller is known to be a major bloom-causing microalga in the southern ocean of the Korean peninsula. The acclimation of this alga to darkness for 10 days was investigated by analyzing the content of various lipids, such as phospholipid (PL), galactolipid (GL), and triacylglyceride (TAG). Actively growing cultures of the alg a under normal growth conditions (14:10 h LD [light:dark] cycle) were transferred to a growth chamber under conditions of no light and no carbon sources in the medium, and the culture was continued for another 10 days. The results showed that the content of TAG and GL decreased gradually during dark incubation, whereas the total BL content changed little; PC, PE, and PG decreased; and PS, PA, and PI increased. An increase in the activity of beta-oxidation and isocitrate lyase (ICL, a glyoxylate cycle enzyme) paralleled the decrease of TAG and GL. These observations strongly suggested that TAG and GL were utilized as alternative carbon sources by the cells under the prolonged dark cultivation. Light treatment of the cells cultivated in the dark for 10 days allowed them to attain the lipid composition that was observed in cells grown in light, These results strongly suggested that the cells maintained their metabolic integrity without unrecoverable cellular damages or cell death during 10 days of dark cultivation.
ISSN
0022-3646
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/6168
DOI
10.1046/j.1529-8817.1999.3520287.x
Bibliographic Citation
JOURNAL OF PHYCOLOGY, v.35, no.2, pp.287 - 292, 1999
Publisher
WILEY
Subject
FATTY-ACID COMPOSITION; BIOCHEMICAL-COMPOSITION; MARINE-PHYTOPLANKTON; ISOCITRATE LYASE; LIPID-METABOLISM; MICROALGAE; TEMPERATURE; IRRADIANCE; INDUCTION; SENESCENT
Keywords
acclimation; beta-oxidation; dark cultivation; isocitrate lyase; membrane lipids; triacyglycerides
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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