Preferential use of acetate over glucose involves acetate-mediated inhibition of glucose uptake during diauxic growth of carrot cells SCIE SCOPUS

Cited 7 time in WEB OF SCIENCE Cited 0 time in Scopus
Title
Preferential use of acetate over glucose involves acetate-mediated inhibition of glucose uptake during diauxic growth of carrot cells
Author(s)
Lee, TK; Lee, SM; Lee, WS
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
1999-10
Abstract
It has been reported that suspension-cultured rice cells grown on mixed carbon sources of glucose (Glc) and acetate exhibited diauxic growth in which acetate was the preferred carbon source (Lee and fee 1996). Carrot (Daucus carota L.) suspension cells, showing a diauxic growth very similar to that of rice cells, were used to delineate the mechanisms underlying this preferential use of acetate over Glc. Uptakes of both Glc and 3-O-methylglucose (3-OMG), a non-metabolizable Glc analogue, were similarly inhibited when acetate or butylate, weak acids which are capable of transporting protons into the cytosol, were present in the uptake assay mixture containing cells harvested during the Glc-utilizing second growth phase. Inhibition of Glc uptake by these weak acids was similar when equivalent experiments were carried out with isolated plasma membranes. It was further shown that Glc uptake, which requires a proper proton gradient across the plasma membranes, was inhibited during the first growth phase by acetate-mediated alkalization of growth medium and/or simultaneous acidification of cytosol. This study strongly suggests that Glc utilization in plant cells is inhibited by co-presenting carbon source(s) which can alter the proton gradient across the plasma membrane.
ISSN
0032-0781
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/6144
DOI
10.1093/oxfordjournals.pcp.a029486
Bibliographic Citation
PLANT AND CELL PHYSIOLOGY, v.40, no.10, pp.1046 - 1052, 1999
Publisher
JAPANESE SOC PLANT PHYSIOLOGISTS
Subject
GENE-EXPRESSION; HIGHER-PLANTS; SUGAR; FUSICOCCIN; TRANSPORT; VULGARIS; PH
Keywords
acetate inhibition; carrot; diauxic growth; glucose uptake
Type
Article
Language
English
Document Type
Article
Publisher
JAPANESE SOC PLANT PHYSIOLOGISTS
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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