Anaerosacchariphilus polymeriproducens gen. nov., sp. nov., an anaerobic bacterium isolated from a salt field

Abstract

A Gram-stain-negative oval-rod-shaped, spore-forming anaerobic bacterium, designated as strain MCWD5(T), was isolated from sediment of a salt pond in the Republic of Korea (35 degrees 7' 18 '' N 126 degrees 19' 4 '' E). The 16S rRNA gene sequence analysis revealed that strain MCWD5(T) had low similarity values to members in the family Lachnospiraceae, such as Robinsoniella peoriensis PPC31(T) (94.8 %), Ruminococcus gauvreauii CCRI-16110(T) (94.2 %) and Lachnotalea glycerinl DLD10(T) (94.0 %), and its phylogenetic position is unstable. The strain could grow at 20-42 degrees C (optimum, 38-42 degrees C), pH 5.5-10.0 (pH 7.0) and with 0-6 % (2.0 %) NaCl. Strain MCWD5(T) could not use nitrate, nitrite, sulfate or sulfite as electron acceptors. The strain could utilize various carbohydrates, such as arabinose, cellobiose, glucose, etc., and polymers such as pectin and starch. The major fatty acids of strain MCWD5 T were C-14:0,C-16:0, C-16:1 omega 7C, C-18:1 omega 7C DMA and summed feature 8 (C-17:1 omega 8c and/or C-17:2), which was clearly different from those of related genera. The major polar lipids were diphosphatidyglycerol, phosphatidyglycerol and an unknown phospholipid. Based on the results of phylogenetic, physiologic and chemotaxonomic studies, Anaerosacchariphilus polymeriproducens gen. nov., sp. nov. with the type strain MCWD5(T) (=KCTC 15595(T) =DSM 105757(T)) is proposed in the family Lachnospiraceae.