Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus
Author(s)
Lee, Min-Chul; Fonseca, Elza; Park, Jun Chul; Yoon, Deok-Seo; Choi, Hyuntae; Kim, Moonkoo; Han, Jeonghoon; Cho, Hyeon-Seo; Shin, Kyung-Hoon; Santos, Miguel L.; Jung, Jee-Hyun; Castro, L. Filipe C.; Lee, Jae-Seong
KIOST Author(s)
Kim, Moon Koo(김문구)Jung, Jee Hyun(정지현)
Publication Year
2019-07-02
Abstract
To examine how tributyltin (TBT), a model obesogen, affects the lipid metabolism in the marine rotifer Brachionus koreanus, we carried out life-cycle studies and determined the in vitro and in silico interactions of retinoid X receptor (RXR) with TBT, the transcriptional levels of RXR and lipid metabolic genes, and the fatty acid content. The lethal concentration 10% (LC10) was determined to be 5.12 mu g/L TBT, and negative effects on ecologically relevant end points (e.g., decreased lifespan and fecundity) were detected at 5 mu g/L TBT. On the basis of these findings, subsequent experiments were conducted below 1 ug/L TBT, which did not show any negative effects on ecologically relevant end points in B. koreanus. Nile red staining analysis showed that after exposure to 1 mu g/L TBT, B. koreanus stored neutral lipids and had significantly increased transcriptional levels of RXR and lipid metabolism-related genes compared to the control. However, the content of total fatty acids did not significantly change at any exposure level. In the single fatty acids profile, a significant increase in saturated fatty acids (SFAs) 14:0 and 20:0 was observed, but the contents of omega-3 and omega-6 fatty acids were significantly decreased. Also, a transactivation assay of TBT with RXR showed that TBT is an agonist of Bk-RXR with a similar fold-induction to the positive control. Taken together, these results demonstrate that TBT-modulated RXR signaling leads to increase in transcriptional levels of lipid metabolism-related genes and the synthesis of SFAs but decreases the content of polyunsaturated fatty acids (PUFAs). Our findings support a wider taxonomic scope of lipid perturbation due to xenobiotic exposure that occurs via NRs in aquatic animals.
ISSN
0013-936X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/573
DOI
10.1021/acs.est.9b01359
Bibliographic Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.53, no.13, pp.7830 - 7839, 2019
Publisher
AMER CHEMICAL SOC
Subject
PERSISTENT ORGANIC POLLUTANTS; ADIPOCYTE DIFFERENTIATION; PEROXISOME PROLIFERATOR; ENDOCRINE DISRUPTION; ORGANOTIN COMPOUNDS; MONOGONONT ROTIFER; ENVIRONMENTAL LEVELS; CALORIC RESTRICTION; SIGNALING-PATHWAY; ADIPOSE-TISSUE
Type
Article
Language
English
Document Type
Article
Publisher
AMER CHEMICAL SOC
Related Researcher
Research Interests

Oil pollution,Antifouling paint,Dissolved methane,유류오염,방오도료,용존메탄

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