Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan SCIE SCOPUS

Cited 13 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan
Author(s)
Hettiarachchi, Sachithra Amarin; Kwon, Young-Kyung; Lee, Youngdeuk; Jo, Eunyoung; Eom, Tae-Yang; Kang, Yoon-Hyeok; Kang, Do-Hyung; De Zoysa, Mahanama; Marasinghe, Svini Dileepa; Oh, Chulhong
KIOST Author(s)
Lee, Young Deuk(이영득)Jo, Eunyoung(조은영)Eom, Tae Yang(엄태양)Kang, Do Hyung(강도형)Marasinghe, Svini Dileepa(Svini)Oh, Chul Hong(오철홍)
Alternative Author(s)
이영득; 조은영; 엄태양; 강윤혁; 강도형; Svini Dileepa Marasinghe; 오철홍
Publication Year
2019-07-08
Abstract
BackgroundAcetyl xylan esterase plays an important role in the complete enzymatic hydrolysis of lignocellulosic materials. It hydrolyzes the ester linkages of acetic acid in xylan and supports and enhances the activity of xylanase. This study was conducted to identify and overexpress the acetyl xylan esterase (AXE) gene revealed by the genomic sequencing of the marine bacterium Ochrovirga pacifica.ResultsThe AXE gene has an 864-bp open reading frame that encodes 287 aa and consists of an AXE domain from aa 60 to 274. Gene was cloned to pET-16b vector and expressed the recombinant AXE (rAXE) in Escherichia coli BL21 (DE3). The predicted molecular mass was 31.75kDa. The maximum specific activity (40.08 U/mg) was recorded at the optimal temperature and pH which were 50 degrees C and pH 8.0, respectively. The thermal stability assay showed that AXE maintains its residual activity almost constantly throughout and after incubation at 45 degrees C for 120min. The synergism of AXE with xylanase on beechwood xylan, increased the relative activity 1.41-fold.ConclusionResulted higher relative activity of rAXE with commercially available xylanase on beechwood xylan showed its potential for the use of rAXE in industrial purposes as a de-esterification enzyme to hydrolyze xylan and hemicellulose-like complex substrates.
ISSN
1475-2859
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/567
DOI
10.1186/s12934-019-1169-y
Bibliographic Citation
MICROBIAL CELL FACTORIES, v.18, 2019
Publisher
BMC
Subject
COLD-ADAPTED ESTERASE; MICROBIAL XYLANASES; FIBROBACTER-SUCCINOGENES; METAGENOMIC LIBRARY; FAMILY; PURIFICATION; DEGRADATION; REVEALS; GENES; SALT
Keywords
Acetyl xylan esterase; Marine bacteria; Ochrovirga pacifica; Synergism; Beech wood xylan
Type
Article
Language
English
Document Type
Article
Publisher
BMC
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