Purification and characterization of an anionic isoperoxidase from scented-geranium callus SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Min, BS | - |
dc.contributor.author | Kim, YK | - |
dc.contributor.author | Ma, CW | - |
dc.contributor.author | Jin, ES | - |
dc.contributor.author | Lee, TK | - |
dc.contributor.author | Lee, JK | - |
dc.contributor.author | Lee, YB | - |
dc.contributor.author | Ryoo, KK | - |
dc.contributor.author | Lee, MY | - |
dc.date.accessioned | 2020-04-20T15:40:31Z | - |
dc.date.available | 2020-04-20T15:40:31Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1082-6068 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/5364 | - |
dc.description.abstract | Secretory anionic isoperoxidase (EC 1.11.1.7), named PA1, was 68-fold purified from scented-geranium (Pelargonium graveolense) callus by using ion exchange chromatography and gel filtration. Isoperoxidase PA1 was a glycoprotein with an isoelectric point (pI) of 4.0. The molecular weight of PA1 was approximately 42.5 and 44 kDa, estimated by SDS PAGE and Sephadex G-150 gel filtration, respectively. The optimum pH of the enzyme was 5.0 for guaiacol and H2O2, and the K-m values for guaiacol and H2O2 were 1.96 and 8.5 mM, respectively. Substrate studies in terms of optimum pHs and K-m values with various synthetic and naturally occurring phenolic compounds were performed. In comparison with cationic isoperoxidase, PC3, which has been already characterized, anionic isoperoxidase PA1 had much lower K-m values for synthetic phenolic compounds and much higher K-m values for naturally occurring phenolic compounds than PC3. Moreover, anionic isoperoxidase PA1 could utilize ferulic acid as a substrate very well, while cationic isoperoxidase PC3 could not utilize ferulic acid as a substrate. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | TAYLOR & FRANCIS INC | - |
dc.subject | LIGNIN PEROXIDASE | - |
dc.subject | STREPTOMYCES-VIRIDOSPORUS | - |
dc.subject | RADISH | - |
dc.subject | CONVERSION | - |
dc.subject | REMOVAL | - |
dc.subject | ACID | - |
dc.title | Purification and characterization of an anionic isoperoxidase from scented-geranium callus | - |
dc.type | Article | - |
dc.citation.endPage | 264 | - |
dc.citation.startPage | 253 | - |
dc.citation.title | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v.34, no.3, pp.253 - 264 | - |
dc.identifier.doi | 10.1081/PB-200026809 | - |
dc.identifier.wosid | 000223804000005 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LIGNIN PEROXIDASE | - |
dc.subject.keywordPlus | STREPTOMYCES-VIRIDOSPORUS | - |
dc.subject.keywordPlus | RADISH | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | REMOVAL | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | anionic isoperoxidase | - |
dc.subject.keywordAuthor | Pelargonium graveolens | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | characterization | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |