Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1 SCIE SCOPUS KCI

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Title
Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1
Author(s)
Lee, Sung-Mok; Lee, Joungmin; Lee, Seong Hyuk; Kim, Jae Young; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Lee, Joungmin(이종민)Lee, Seong Hyuk(이성혁)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2019-09
Abstract
Hydrogen has been receiving considerable attention as a clean energy carrier. Thermococcus onnurineus NA1, a hyperthermophilic archaeon, is capable of producing hydrogen using carbon monoxide (CO) in a growth-associated manner, providing a promising platform to upcycle industrial waste gases. In order to improve cost-effectiveness of the biological hydrogen production from carbon monoxide (CO), we developed formulation of a low-cost medium for T. onnurineus 156T by substituting fishmeal and sea salt for yeast extract (YE) and reagent-grade salts, respectively. In serum bottle cultures, hydrogen production by the 156T strain was less than 30% in fishmeal-containing media when compared to that of the YE-containing MM1 medium. However, hydrogen production was increased substantially by adaptive evolution in the fishmeal-sea salt (FMSS) medium. In bioreactor experiments, the resulting strain 156T-FA showed shorter lag phase and 70% higher maximum hydrogen production rate in FMSS medium than the parent strain 156T. Our results showed that the H-2 production cost was reduced by ca. 95%, when YE and chemical salts were replaced by fishmeal and sea salt, respectively.
ISSN
1226-8372
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/535
DOI
10.1007/s12257-019-0240-1
Bibliographic Citation
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.24, no.5, pp.833 - 838, 2019
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Subject
H-2 PRODUCTION; BIOHYDROGEN PRODUCTION; GROWTH; FUEL
Keywords
hydrogen; carbon monoxide (CO); medium cost; Thermococcus onnurineus NA1; adaptive evolution
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Related Researcher
Research Interests

Marine extremophile,해양극한미생물

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