Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1
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Title
- Formulation of a Low-cost Medium for Improved Cost-effectiveness of Hydrogen Production by Thermococcus onnurineus NA1
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Author(s)
- Lee, Sung-Mok; Lee, Joungmin; Lee, Seong Hyuk; Kim, Jae Young; Lee, Hyun Sook; Kang, Sung Gyun
- KIOST Author(s)
- Lee, Sung Mok(이성목); Lee, Joungmin(이종민); Lee, Seong Hyuk(이성혁); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균)
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Alternative Author(s)
- 이성목; 이종민; 이성혁; 김재영; 이현숙; 강성균
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Publication Year
- 2019-09
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Abstract
- Hydrogen has been receiving considerable attention as a clean energy carrier. Thermococcus onnurineus NA1, a hyperthermophilic archaeon, is capable of producing hydrogen using carbon monoxide (CO) in a growth-associated manner, providing a promising platform to upcycle industrial waste gases. In order to improve cost-effectiveness of the biological hydrogen production from carbon monoxide (CO), we developed formulation of a low-cost medium for T. onnurineus 156T by substituting fishmeal and sea salt for yeast extract (YE) and reagent-grade salts, respectively. In serum bottle cultures, hydrogen production by the 156T strain was less than 30% in fishmeal-containing media when compared to that of the YE-containing MM1 medium. However, hydrogen production was increased substantially by adaptive evolution in the fishmeal-sea salt (FMSS) medium. In bioreactor experiments, the resulting strain 156T-FA showed shorter lag phase and 70% higher maximum hydrogen production rate in FMSS medium than the parent strain 156T. Our results showed that the H-2 production cost was reduced by ca. 95%, when YE and chemical salts were replaced by fishmeal and sea salt, respectively.
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ISSN
- 1226-8372
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/535
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DOI
- 10.1007/s12257-019-0240-1
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Bibliographic Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.24, no.5, pp.833 - 838, 2019
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Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
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Keywords
- hydrogen; carbon monoxide (CO); medium cost; Thermococcus onnurineus NA1; adaptive evolution
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Type
- Article
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Language
- English
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Document Type
- Article
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