Dissemination of transferable AmpC-type beta-lactamase (CMY-10) in a Korean hospital SCIE SCOPUS

Cited 19 time in WEB OF SCIENCE Cited 21 time in Scopus
Title
Dissemination of transferable AmpC-type beta-lactamase (CMY-10) in a Korean hospital
Author(s)
Lee, JH; Jung, HI; Jung, JH; Park, JS; Ahn, JB; Jeong, SH; Jeong, BC; Lee, JH; Lee, SH
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2004-09
Abstract
To determine dissemination and genotype of AmpC beta-lactamases and an extended-spectrum beta-lactamase among clinical isolates of Enterobacteriaceae, we performed antibiotic susceptibility testing, pI determination, induction test, plasmid profiles, transconjugation test, enterobacterial repetitive consensus (ERIC)-PCR, and DNA sequencing. Among the 51 clinical isolates collected from a university hospital in Korea, six isolates were resistant to cephamycins. All six isolates produced a plasmid-encoded AmpC-type beta-lactamase, CMY-10. Five strains also produced one or more other beta-lactamases: SHV-12, an extended-spectrum beta-lactamase (five isolates); TEM-1, a class A beta-lactamase (two isolates); and a chromosomal AmpC beta-lactamase (one isolate, a strain of Enterobacter aerogenes, which produced all four of the beta-lactamases that were identified). One of six isolates produced only CMY-10. ERIC-PCR analysis revealed that dissemination of CMY-10 and SHV-12 was due to a clonal outbreak of a resistant strain and to the interspecies spread of resistance to cephamycins and broad-spectrum beta-lactams in Korea. CMY-10 beta-lactamase genes that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized from six clinical isolates. A sequence identical to the common regions in In6, In7, and a novel integron from pSAL-1 was found upstream from bla(CMY-10) gene at nucleotides 1-71. A total of 15 nucleotides (I-15) or 18 nucleotides (I-18) between position 71 and 72 were inserted into the bla(CMY-10) gene. The bla(CMY-10) gene might be inserted into a Bull-type complex integron by I-15 or I-18.
ISSN
1076-6294
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/5173
DOI
10.1089/1076629041939319
Bibliographic Citation
MICROBIAL DRUG RESISTANCE, v.10, no.3, pp.224 - 230, 2004
Publisher
MARY ANN LIEBERT, INC
Type
Article
Language
English
Document Type
Article
Publisher
MARY ANN LIEBERT, INC
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