Cloning, expression, and characterization of a DNA ligase from a hyperthermophilic archaeon Thermococcus sp. SCIE SCOPUS
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- Title
- Cloning, expression, and characterization of a DNA ligase from a hyperthermophilic archaeon Thermococcus sp.
- KIOST Author(s)
- Kim, Yun Jae(김윤재); Lee, Hyun Sook(이현숙); Yang, Sung Hyun(양성현); Lim, Jae Kyu(임재규); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현)
- Alternative Author(s)
- 김윤재; 이현숙; 배승섭; 전정호; 양성현; 임재규; 강성균; 이정현
- Publication Year
- 2006-03
- Abstract
- Genomic analysis of a hyperthermophilic archaeon, Thermococcus sp. NA1, revealed an ORF of 1689 bases encoding 562 amino acids that showed a high similarity to DNA ligases from other hyperthermophilic archaea. The ligase, which was designated TNA1_ lig ( Thermococcus sp. NA1 ligase), was cloned and expressed in Escherichia coli. The recombinant TNA1_ lig was purified by metal affinity chromatography. The optimum ligase activity of the recombinant TNA1_ lig occurred at 80 degrees C and pH 7.5. The enzyme was activated by MgCl2 and ZnCl2 but was inhibited by MnCl2 and NiCl2. Additionally, the enzyme was activated by either ATP or NAD(+).
- ISSN
- 0141-5492
- Bibliographic Citation
- BIOTECHNOLOGY LETTERS, v.28, no.6, pp.401 - 407, 2006
- Publisher
- SPRINGER
- Subject
- FUNCTIONAL DOMAINS; PURIFICATION; REPAIR; GENE
- Keywords
- Archaea; DNA ligase; Thermococcus sp.
- Type
- Article
- Language
- English
- Document Type
- Article
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- There are no files associated with this item.
