Psychroserpens mesophilus sp nov., a mesophilic marine bacterium belonging to the family Flavobacteriaceae isolated from a young biofilm

Abstract

A number of marine bacteria isolated from young biofilms were characterized as belonging to the family Flavobacteriaceae. The taxonomic characterization of strain KOPRI 13649(T), which was isolated from an acrylic surface at the seashore at Gangneung, Korea, is reported here. The nearly complete 16S rRNA gene sequence of strain KOPRI 13649(T) was determined and was found to have a high level of similarity with that of Psychroserpens burtonensis (95.0-95.6%). In addition, phylogenetic analysis and comparison with closely related strains confirmed that the strain represented a novel member of the genus Psychroserpens. The major respiratory quinone of strain KOPRI 13649(T) was MK-6 and the DNA G + C content was 29.8 mol%. The dominant fatty acid methyl esters were i-15: 0, a-15: 0, i-16: 0, i-15: 1 omega 10, 16: 1 omega 7 and 15: 0. Growth was observed at 10-34 degrees C (optimum 30 degrees C), at pH 6-9 (optimum 6.5-8.0) and with 0.5-4% NaCl (optimum 1%). On the basis of the polyphasic taxonomic evidence presented, strain KOPRI 13649(T) (= KCCM 4226(T) = jCM 13413(T)) should be classified as the type strain of a novel species in the genus Psychroserpens, for which the name Psychroserpens mesophilus sp. nov. is proposed.