Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli) SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Jung, Jee-Hyun | - |
dc.contributor.author | Shim, Won Joon | - |
dc.contributor.author | Addison, R. F. | - |
dc.contributor.author | Baek, Jae Min | - |
dc.contributor.author | Han, Chang-Hee | - |
dc.date.accessioned | 2020-04-20T12:55:37Z | - |
dc.date.available | 2020-04-20T12:55:37Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2006-06 | - |
dc.identifier.issn | 1532-0456 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/4883 | - |
dc.description.abstract | To investigate the in vivo estrogenic activity of 4-nonylphenol (4-NP) on reproductive function, we have measured the expression level of rockfish vitellogenin (rVTG) mRNA, concentration of plasma VTG and levels of plasma estradiol-17 beta(E-2) and testosterone (T) by radioimmunoassay (RIA). An rVTG mRNA transcript of approximately 4.0kb was extracted from hepatic tissue for Northern blot analysis, and the effects of 4-NP on rVTG mRNA expression in vivo with both male and female juvenile rockfish were examined. The level of rVTG mRNA expression was increased 48b after injection with 4-NP of 10mg/kg body mass and after injection in both male and female rockfish. The level of rVTG mRNA expression was increased 24h after injection in male rockfish injected with 4-NP of 25mg/kg body mass, while the level of rVTG mRNA expression was increased 12 It after injection in female injected with same dosage. Concentrations of plasma rVTG in female injected with 4-NP of 10 and 25mg/kg b.w. were increased 72h after injection and reached 35 and 66mg/mL, respectively. In male, rVTG concentrations with the dosage of 25mg/kg body mass were increased 72h after injection and reached to 14 and 65mg/mL at 168h, respectively. Plasma concentrations of T and E-2 in female and male injected with 4-NP were not significantly different between two 4-NP dosages. These results suggest that 4-NP may disrupt the reproductive system of immature rockfish by acting directly on vitellogenesis. Immature females injected with 4-NP were more sensitive than immature males, but VTG in immature males is likely to return to normal conditions slowly. There are obviously large interspecies differences in response of fish to 4-NP. (c) 2006 Elsevier Inc. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | TROUT ONCORHYNCHUS-MYKISS | - |
dc.subject | VITELLOGENIN MESSENGER-RNA | - |
dc.subject | MALE SHEEPSHEAD MINNOWS | - |
dc.subject | RAINBOW-TROUT | - |
dc.subject | CYPRINODON-VARIEGATUS | - |
dc.subject | SPATIAL-DISTRIBUTION | - |
dc.subject | ESTROGENIC COMPOUNDS | - |
dc.subject | PIMEPHALES-PROMELAS | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | ZONA-RADIATA | - |
dc.title | Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli) | - |
dc.type | Article | - |
dc.citation.endPage | 170 | - |
dc.citation.startPage | 162 | - |
dc.citation.title | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | - |
dc.citation.volume | 143 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 정지현 | - |
dc.contributor.alternativeName | 심원준 | - |
dc.identifier.bibliographicCitation | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.143, no.2, pp.162 - 170 | - |
dc.identifier.doi | 10.1016/j.cbpc.2006.01.009 | - |
dc.identifier.scopusid | 2-s2.0-33646490989 | - |
dc.identifier.wosid | 000237836300004 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ESTROGENIC COMPOUNDS | - |
dc.subject.keywordPlus | PIMEPHALES-PROMELAS | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | ZONA-RADIATA | - |
dc.subject.keywordPlus | TROUT ONCORHYNCHUS-MYKISS | - |
dc.subject.keywordPlus | VITELLOGENIN MESSENGER-RNA | - |
dc.subject.keywordPlus | MALE SHEEPSHEAD MINNOWS | - |
dc.subject.keywordPlus | RAINBOW-TROUT | - |
dc.subject.keywordPlus | CYPRINODON-VARIEGATUS | - |
dc.subject.keywordPlus | SPATIAL-DISTRIBUTION | - |
dc.subject.keywordAuthor | vitellogenin (VTG) | - |
dc.subject.keywordAuthor | rockfish | - |
dc.subject.keywordAuthor | Sebastes schlegeli | - |
dc.subject.keywordAuthor | testosterone | - |
dc.subject.keywordAuthor | estradiol-17 beta | - |
dc.subject.keywordAuthor | 4-NP | - |
dc.subject.keywordAuthor | radioimmunoassay (RIA) | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalResearchArea | Zoology | - |