Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Jung, Jee-Hyun -
dc.contributor.author Shim, Won Joon -
dc.contributor.author Addison, R. F. -
dc.contributor.author Baek, Jae Min -
dc.contributor.author Han, Chang-Hee -
dc.date.accessioned 2020-04-20T12:55:37Z -
dc.date.available 2020-04-20T12:55:37Z -
dc.date.created 2020-01-28 -
dc.date.issued 2006-06 -
dc.identifier.issn 1532-0456 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4883 -
dc.description.abstract To investigate the in vivo estrogenic activity of 4-nonylphenol (4-NP) on reproductive function, we have measured the expression level of rockfish vitellogenin (rVTG) mRNA, concentration of plasma VTG and levels of plasma estradiol-17 beta(E-2) and testosterone (T) by radioimmunoassay (RIA). An rVTG mRNA transcript of approximately 4.0kb was extracted from hepatic tissue for Northern blot analysis, and the effects of 4-NP on rVTG mRNA expression in vivo with both male and female juvenile rockfish were examined. The level of rVTG mRNA expression was increased 48b after injection with 4-NP of 10mg/kg body mass and after injection in both male and female rockfish. The level of rVTG mRNA expression was increased 24h after injection in male rockfish injected with 4-NP of 25mg/kg body mass, while the level of rVTG mRNA expression was increased 12 It after injection in female injected with same dosage. Concentrations of plasma rVTG in female injected with 4-NP of 10 and 25mg/kg b.w. were increased 72h after injection and reached 35 and 66mg/mL, respectively. In male, rVTG concentrations with the dosage of 25mg/kg body mass were increased 72h after injection and reached to 14 and 65mg/mL at 168h, respectively. Plasma concentrations of T and E-2 in female and male injected with 4-NP were not significantly different between two 4-NP dosages. These results suggest that 4-NP may disrupt the reproductive system of immature rockfish by acting directly on vitellogenesis. Immature females injected with 4-NP were more sensitive than immature males, but VTG in immature males is likely to return to normal conditions slowly. There are obviously large interspecies differences in response of fish to 4-NP. (c) 2006 Elsevier Inc. All rights reserved. -
dc.description.uri 1 -
dc.language English -
dc.publisher ELSEVIER SCIENCE INC -
dc.subject TROUT ONCORHYNCHUS-MYKISS -
dc.subject VITELLOGENIN MESSENGER-RNA -
dc.subject MALE SHEEPSHEAD MINNOWS -
dc.subject RAINBOW-TROUT -
dc.subject CYPRINODON-VARIEGATUS -
dc.subject SPATIAL-DISTRIBUTION -
dc.subject ESTROGENIC COMPOUNDS -
dc.subject PIMEPHALES-PROMELAS -
dc.subject MOLECULAR-CLONING -
dc.subject ZONA-RADIATA -
dc.title Protein and gene expression of VTG in response to 4-nonylphenol in rockfish (Sebastes schlegeli) -
dc.type Article -
dc.citation.endPage 170 -
dc.citation.startPage 162 -
dc.citation.title COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY -
dc.citation.volume 143 -
dc.citation.number 2 -
dc.contributor.alternativeName 정지현 -
dc.contributor.alternativeName 심원준 -
dc.identifier.bibliographicCitation COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v.143, no.2, pp.162 - 170 -
dc.identifier.doi 10.1016/j.cbpc.2006.01.009 -
dc.identifier.scopusid 2-s2.0-33646490989 -
dc.identifier.wosid 000237836300004 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus ESTROGENIC COMPOUNDS -
dc.subject.keywordPlus PIMEPHALES-PROMELAS -
dc.subject.keywordPlus MOLECULAR-CLONING -
dc.subject.keywordPlus ZONA-RADIATA -
dc.subject.keywordPlus TROUT ONCORHYNCHUS-MYKISS -
dc.subject.keywordPlus VITELLOGENIN MESSENGER-RNA -
dc.subject.keywordPlus MALE SHEEPSHEAD MINNOWS -
dc.subject.keywordPlus RAINBOW-TROUT -
dc.subject.keywordPlus CYPRINODON-VARIEGATUS -
dc.subject.keywordPlus SPATIAL-DISTRIBUTION -
dc.subject.keywordAuthor vitellogenin (VTG) -
dc.subject.keywordAuthor rockfish -
dc.subject.keywordAuthor Sebastes schlegeli -
dc.subject.keywordAuthor testosterone -
dc.subject.keywordAuthor estradiol-17 beta -
dc.subject.keywordAuthor 4-NP -
dc.subject.keywordAuthor radioimmunoassay (RIA) -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalWebOfScienceCategory Endocrinology & Metabolism -
dc.relation.journalWebOfScienceCategory Toxicology -
dc.relation.journalWebOfScienceCategory Zoology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Endocrinology & Metabolism -
dc.relation.journalResearchArea Toxicology -
dc.relation.journalResearchArea Zoology -
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