Heavy metal-induced differential gene expression of metallothionein in Javanese medaka, Oryzias javanicus
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Title
- Heavy metal-induced differential gene expression of metallothionein in Javanese medaka, Oryzias javanicus
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Author(s)
- Woo, Seonock; Yum, Seungshic; Jung, Jee Hyun; Shim, Won Joon; Lee, Chang-Hoon; Lee, Taek-Kyun
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Yum, Seung Shic(염승식); Jung, Jee Hyun(정지현); Shim, Won Joon(심원준); Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 우선옥; 염승식; 정지현; 심원준; 이택견
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Publication Year
- 2006-11
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Abstract
- A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)(+) stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed via real-time quantitative polymerase chain reaction (PCR). The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.
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ISSN
- 1436-2228
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4830
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DOI
- 10.1007/s10126-006-6046-0
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Bibliographic Citation
- MARINE BIOTECHNOLOGY, v.8, no.6, pp.654 - 662, 2006
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Publisher
- SPRINGER
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Subject
- MESSENGER-RNA EXPRESSION; MYTILUS-EDULIS; EXPOSURE; BIOMARKER; CADMIUM; INDUCTION; COPPER; FISH; QUANTIFICATION; SEQUENCE
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Keywords
- heavy metals; Javanese medaka; metallothionein; OjaMT; Oryzias javanicus; real-time quantitative PCR
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Type
- Article
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Language
- English
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Document Type
- Article
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