Heavy metal-induced differential gene expression of metallothionein in Javanese medaka, Oryzias javanicus SCIE SCOPUS

Cited 59 time in WEB OF SCIENCE Cited 69 time in Scopus
Title
Heavy metal-induced differential gene expression of metallothionein in Javanese medaka, Oryzias javanicus
Author(s)
Woo, Seonock; Yum, Seungshic; Jung, Jee Hyun; Shim, Won Joon; Lee, Chang-Hoon; Lee, Taek-Kyun
KIOST Author(s)
Woo, Seon Ock(우선옥)Yum, Seung Shic(염승식)Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)Lee, Taek Kyun(이택견)
Alternative Author(s)
우선옥; 염승식; 정지현; 심원준; 이택견
Publication Year
2006-11
Abstract
A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)(+) stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed via real-time quantitative polymerase chain reaction (PCR). The level of hepatic OjaMT mRNA was increased in a dose-dependent manner by Ag, Cd, Cu, and Zn after 24 h of exposure. However, after Cr and Ni exposure, a significant decrease in OjaMT levels was observed. Cadmium-induced OjaMT expression was detectable in fishes as young as 3 months. After Cd exposure, OjaMT induction was prominent in intestine and liver and moderate in muscle and gill. OjaMT mRNA levels could represent a good biomarker for monitoring heavy metals in seawater.
ISSN
1436-2228
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4830
DOI
10.1007/s10126-006-6046-0
Bibliographic Citation
MARINE BIOTECHNOLOGY, v.8, no.6, pp.654 - 662, 2006
Publisher
SPRINGER
Subject
MESSENGER-RNA EXPRESSION; MYTILUS-EDULIS; EXPOSURE; BIOMARKER; CADMIUM; INDUCTION; COPPER; FISH; QUANTIFICATION; SEQUENCE
Keywords
heavy metals; Javanese medaka; metallothionein; OjaMT; Oryzias javanicus; real-time quantitative PCR
Type
Article
Language
English
Document Type
Article
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