Activation of the nucleotide excision repair pathway by crude oil exposure: A translational study from model organisms to the Hebei Spirit Oil Spill Cohort SCIE SCOPUS

Cited 2 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Activation of the nucleotide excision repair pathway by crude oil exposure: A translational study from model organisms to the Hebei Spirit Oil Spill Cohort
Author(s)
Kim, Youngho; Jeong, Jaeseong; Chatterjee, Nivedita; Yim, Un Hyuk; Kwon, Jung-Hwan; Park, Myung-Sook; Choi, Jinhee
KIOST Author(s)
Yim, Un Hyuk(임운혁)
Alternative Author(s)
임운혁
Publication Year
2019-11
Abstract
In order to gain insight into the human health implications of the Hebei Spirit Oil Spill (HSOS), the mechanism of toxicity of the Iranian heavy crude (IHC), the main oil component in the HSOS was investigated in Caenorhabditis elegans and zebrafish. The identified mechanism was translated to humans using blood samples from Taean residents, who experienced HSOS with different levels of exposure to the spill. C. elegans TF RNAi screening with IHC oil revealed the nucleotide excision repair (NER) pathway as being significantly involved by oil exposure. To identify the main toxicity contributors within the chemical mixture of the crude oil, further studies were conducted on C elegans by exposure to C3-naphthalene, an alkylated polycyclic aromatic hydrocarbon (PAH), which constitutes one of the major components of IHC oil. Increased expression of NER pathway genes was observed following exposure to the IHC oil, C3-naphthalene enriched fraction and C3-naphthalene. As the NER pathway is conserved in fish and humans, the same experiment was conducted in zebrafish, and the data were similar to what was seen in C elegans. Increased expression of NER pathway genes was observed in human samples from the high exposure group, which suggests the involvement of the NER pathway in IHC oil exposure. Overall, the study suggests that IHC oil may cause bulk damage to DNA and activation of the NER system and Alkylated PAHs are the major contributor to DNA damage. Our study provides an innovative approach for studying translational toxicity testing from model organisms to human health. (C) 2019 Elsevier Ltd. All rights reserved.
ISSN
0269-7491
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/477
DOI
10.1016/j.envpol.2019.112997
Bibliographic Citation
ENVIRONMENTAL POLLUTION, v.254, 2019
Publisher
ELSEVIER SCI LTD
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; ENDOCRINE-DISRUPTION POTENTIALS; OXIDATIVE STRESS BIOMARKERS; TOXICITY; SEDIMENTS; RECONNAISSANCE; COMMUNITIES; ZEBRAFISH; RESIDENTS; CHILDREN
Keywords
Caenorhabditis elegans; Zebrafish; Hebei Spirit Oil Spill accident; Translational approach; Nucleotide excision repair system
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
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