Indole-6-carboxaldehyde isolated from Sargassum thunbergii inhibits the expression and secretion of matrix metalloproteinase-9 SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Indole-6-carboxaldehyde isolated from Sargassum thunbergii inhibits the expression and secretion of matrix metalloproteinase-9
Author(s)
Kim, Tae-Hee; Heo, Soo-Jin; Ko, Seok-Chun; Park, Won Sun; Choi, Il-Whan; Yi, Myunggi; Jung, Won-Kyo
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2019-11
Abstract
Sargassum thunbergii is a brown alga from which various bioactive compounds can be extracted. Among these, the activities of indole derivatives, particularly as potential inhibitors of matrix metalloproteinases (MMPs), and their underlying mechanisms have been rarely investigated. Therefore, we evaluated the inhibitory effects of indole-6-car-boxaldehyde (I6CA) on MMP-9 by gelatin zymography and western blot anlaysis. We used phorbol 12-myristate 13-acetate (PMA), which is known to induce MMP-9 expression and secretion, to stimulate HT1080 cells. Our results revealed that I6CA significantly inhibited MMP-9 expression and secretion, without significantly affecting the viability of PMA-stimulated HT1080 cells. Our mechanistic studies indicated that I6CA suppressed the phosphorylation and activation of two mitogen-activated protein kinases (MAPKs), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase 1/2 (ERK). Furthermore, I6CA inhibited the phosphorylation of inhibitor of kappa B alpha (I kappa B alpha) in response to PMA stimulation, which suppressed nuclear factor-kappa B (NF-kappa B) p65 subunit nuclear translocation. Collectively, I6CA was determined to suppress MMP-9 expression and secretion, and effects were proposed to be mediated via the inhibition of the MAPK and NF-kappa B p65 pathways. Therefore, we suggested I6CA to be a potential therapeutic agent for MMP-9-related processes, including tumor invasion and metastasis; however, further investigation is required.
ISSN
1107-3756
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/475
DOI
10.3892/ijmm.2019.4319
Bibliographic Citation
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.44, no.5, pp.1979 - 1987, 2019
Publisher
SPANDIDOS PUBL LTD
Subject
NF-KAPPA-B; MARINE BROWN ALGA; MATRIX METALLOPROTEINASES; CELL INVASION; IN-VITRO; ANTIINFLAMMATORY ACTION; MMP-9 EXPRESSION; GENE-EXPRESSION; UP-REGULATION; CANCER
Keywords
Sargassum thunbergii; indole-6-carboxaldehyde; matrix metalloproteinase-9 (MMP-9); mitogen-activated protein kinases; nuclear factor-kappa B
Type
Article
Language
English
Document Type
Article
Publisher
SPANDIDOS PUBL LTD
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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