Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos SCIE SCOPUS

Cited 17 time in WEB OF SCIENCE Cited 16 time in Scopus
Title
Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos
Author(s)
Jung, Jee-Hyun; Addison, R. F.; Shim, Won Joon
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2007-06
Abstract
Cholinesterases (ChEs) have been characterized in marbled sole (Limanda yokohamae) for use as a possible biomarker of pollution exposure. In brain, ChEs existed almost exclusively (> 95%) as acetylcholinesterase (AChE) whereas in muscle, about 20-30% of ChE activity was in the form of butyrylcholinesterase (BChE; pseudocholinesterase). Acetylthiocholine and butyrylthiocholine (identified in mammalian studies as diagnostic substrates for AChE and BChE respectively) were hydrolyzed mainly, but not exclusively, by these enzymes. The inhibitors BW284C51 and iso-OMPA (identified in mammalian studies as diagnostic inhibitors of AChE and BChE respectively) were not specific for these enzymes in marbled sole. Brain AChE and muscle AChE and BChE were characterized in terms of their kinetic properties (K-M etc.) and optimal conditions (substrate concentration, protein concentration, pH etc.) were established to allow routine assays of ChE activity to proceed under pseudo-first order conditions. The sensitivity of ChEs to a locally significant pesticide, iprobenfos (IBP; kitazin) was established in terms Of IC50 concentrations. Brain AChE was relatively insensitive to IBP, but muscle AChE and BChE were sensitive to IBP concentrations in the high nM range. However, ambient IBP concentrations in Korean coastal waters are usually not high enough to cause detectable ChE inhibition in this species. (c) 2007 Elsevier Ltd. All rights reserved.
ISSN
0141-1136
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4690
DOI
10.1016/j.marenvres.2006.12.007
Bibliographic Citation
MARINE ENVIRONMENTAL RESEARCH, v.63, no.5, pp.471 - 478, 2007
Publisher
ELSEVIER SCI LTD
Subject
NEUROTOXIC CONTAMINATION; ACETYLCHOLINESTERASE; FLOUNDER; ACCUMULATION; PESTICIDES; BIOMARKER; KOREA; FISH; BAY; IBP
Keywords
acetylcholinesterase (AChE); butyryleholinesterase (BCchE); marbled sole; Limanda yokohaniae; iprobenfos; IBP; kitazin
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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