A synergistic therapy against influenza virus A/H1N1/PR8 by a HA1 specific neutralizing single-domain VL and an RNA hydrolyzing scFv SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Hoang, Phuong Thi | - |
dc.contributor.author | Luong, Quynh Xuan Thi | - |
dc.contributor.author | Ayun, Ramadhani Qurrota | - |
dc.contributor.author | Lee, Yongjun | - |
dc.contributor.author | Oh, Kwang-Ji | - |
dc.contributor.author | Kim, Taehyun | - |
dc.contributor.author | Lee, Taek Kyun | - |
dc.contributor.author | Lee, Sukchan | - |
dc.date.accessioned | 2024-04-24T02:30:08Z | - |
dc.date.available | 2024-04-24T02:30:08Z | - |
dc.date.created | 2024-04-22 | - |
dc.date.issued | 2024-04 | - |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/45530 | - |
dc.description.abstract | The emergence of anti-influenza drug-resistant strains poses a challenge for influenza therapy due to mutations in the virus’s surface protein. Recently, there has been increasing interest in combination therapy consisting of two or more drugs as a potential alternative approach, aiming to enhance therapeutic efficacy. In this study, we investigated a novel synergistic therapy with a vertical effect using a single-domain VL-HA1-specific antibody against H1N1/PR8 and a horizontal effect using an RNA catalytic antibody with broad-spectrum influenza antiviral drug. We isolated a single-domain VL-HA1-specific (NVLH8) antibody binding to the virus particles showing a neutralizing activity against influenza virus A, specifically H1N1/PR8, as determined by the reduction in plaque number and lower viral HA protein expression in vitro. The neutralizing antibody likely prevented the viral entry, specifically at the viral genome-releasing step. Additionally, the 3D8 scFv hydrolyzed viral RNAs in the cytoplasm, including mRNA, vRNA, and cRNA in MDCK cells. The combined treatment of neutralizing antibodies for a vertical effect and 3D8 scFv for a horizontal effect produced a synergistic effect providing a novel approach against viral diseases when compared with a single treatment. Our results indicated that combining treatment, in particular two proteins exhibiting different mechanisms of action increased the antiviral activity against the influenza virus. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Frontiers Media S.A. | - |
dc.title | A synergistic therapy against influenza virus A/H1N1/PR8 by a HA1 specific neutralizing single-domain VL and an RNA hydrolyzing scFv | - |
dc.type | Article | - |
dc.citation.title | Frontiers in Microbiology | - |
dc.citation.volume | 15 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | Frontiers in Microbiology, v.15 | - |
dc.identifier.doi | 10.3389/fmicb.2024.1355599 | - |
dc.identifier.scopusid | 2-s2.0-85192069744 | - |
dc.identifier.wosid | 001214736600001 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordAuthor | neutralizing antibodies | - |
dc.subject.keywordAuthor | bio-panning | - |
dc.subject.keywordAuthor | synergistic effect | - |
dc.subject.keywordAuthor | influenza virus | - |
dc.subject.keywordAuthor | 3D8 scFv | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |