시화호에서 채집한 풀망둑 Acanthogobius hasta의 간장 약물대사효소계 및 항산화계의 반응

Abstract

The aim of this study was to assess the responses of mixed function oxygenase(MFO)and antioxidative systems of feral Javelin goby, Acanthogobius hasta, caught in two sites ofdifferent pollution level in Shihwa lake, which has been a highly polluted lake by organic pollu-tants from nearby industrial complexes and sites.Enzymes analyzed in phase I of MFO system are cytochrome P450(CYP), NADPH-cytochromeP450 reductase (P450R), NADH-cytochrome b5 reductase (b5R), and ethoxyresorufin deethylase(EROD). Phase II enzyme of glutathione S-transferase (GST) in MFO system was also investi-gated. Moreover, oxidative-enzyme system including catalase (CAT), glutathione reductase (GR)and total-glutathione peroxidase (GPX) activities and glutathione concentration in both of oxi-dized(GSSG) and reduced form(GSH) were determined.P450R, b5R, and GST activities of fish are relatively high in the polluted area, whereas hepaticEROD activity levels of fish in polluted area were lower than those of unpolluted area. CYPconcentrations are not different between areas. These results indicated that feral Acanthogobiushastawere adaptive to highly polluted environment and exposed to oxidative stress in Shihwa lake.